2株内生真菌对菊花抗旱特性的影响  

Effect of PEG stress on plantlets of Chrysanthemum morifolium induced by endophytic Botrytis sp. (C1) and Chaetomium globosum(C4)

作　　者：宋文玲[1] 刘晓珍[1] 蔡信之[2] 孙迪[1] 戴传超[1] 

机构地区：[1]江苏省微生物资源产业化工程技术研究中心江苏省生物多样性与生物技术重点实验室南京师范大学生命科学学院,江苏南京210046 [2]盐城师范学院江苏省滩涂生物资源与环境保护重点实验室,江苏盐城224002

出　　处：《中国中药杂志》2011年第3期302-306,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30770073);江苏省滩涂生物资源与环境保护重点实验室开放项目(JLCBE07005)

摘　　要：目的:以PEG6000模拟干旱条件,研究接种内生真菌(葡萄孢属C1菌Botrytissp.、球毛壳菌C4菌Chaetomium globosum对药用菊花Ch.morifolium抗旱性的影响。方法:分别用0%,10%,20%,30%,40%PEG6000胁迫菊花组培苗4 d,测定各处理组菊花生物量,叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性及叶片丙二醛(MDA)、可溶性蛋白含量。结果:模拟干旱胁迫后,接种内生真菌的菊花长势好于对照(未接菌),PEG6000胁迫4 d后,随着胁迫浓度的增加,菊花总生物量不断减少,接菌组生物量显著高于对照,C4组高于C1组;各处理组MDA含量随着胁迫浓度的增加而不断增加;各处理组POD活性、可溶性蛋白含量随着胁迫浓度的增加均呈现先增加后减小的趋势;对照SOD活性随着胁迫浓度的增加而逐渐增加,接菌组SOD活性基本保持不变;对照PAL活性随着胁迫浓度的增加而逐渐增加。不同浓度PEG胁迫后,接菌组MDA含量始终低于对照,SOD活性、POD活性、可溶性蛋白含量、PAL活性均始终高于对照。结论:接种内生真菌提高菊花的抗旱能力。The effect of the endophytic fungi Botrytis sp. （Cl） or Chaetomium globosum （CA） on the drought resistance of Chrysanthemum morifolium was studied. Ch. morifolium plantlets were inoculated with Cl, CA and cultured in the pots for 60 days, then the plantlets were stressed by 0%, 10% , 20%, 30%, 40% PEG6000 respectively in order to simulate different drought condi- tions. Biomass, the activities of SOD, POD, PAL, the contents of MDA and soluble protein of each group were determined. The re- suits showed that endophytic fungi groups grew better than the control （without inoculation endophytic fungi）. With the increasing of the concentration of PEG6000, the biomass of Ch. morifolium of each groups decreased, while the biomass of fungi groups was signifi- cantly higher than that of control, moreover CA group higher than C1 group. With the concentration of PEG increasing, the content of MDA of each group increased too, while POD activity and soluble protein content of all treatments increased at first and then de- creased. SOD activity and PAL activity of the control were increased with the increase of PEG concentration, but SOD activity of the two fungi groups were stable. After been stressed by different concentrations of PEG, MDA content of two fungi groups were always lower than the control, while SOD activity, POD activity, PAL activity and soluble protein content were higher. In conclusion, endophytic fungi can increase the drought resistance of Ch. morifolium.

关 键 词：PEG6000 葡萄孢菌C1菌株 球毛壳菌C4菌株 菊花 抗旱性 抗氧化 

分 类 号：S567.239[农业科学—中草药栽培]