广西尖吻蝮蛇毒K组分抗血管生成活性研究  

The Anti-angiogenesis Activity of Fraction K Purified from Guangxi Agkitrodon Acutus Venom

作　　者：李映新[1] 李剑军[2] 周先丽[3] 雷丹青[4] 

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西壮族自治区疾病预防控制中心,南宁530028 [3]桂林医学院,541004 [4]广西医科大学蛇毒研究所,南宁530021

出　　处：《医药导报》2011年第2期157-159,共3页Herald of Medicine

基　　金：广西壮族自治区自然科学基金资助项目(基金编号:0575062)

摘　　要：目的探讨广西尖吻蝮蛇毒小分子多肽组分(K组分)对血管生成的抑制作用。方法采用噻唑蓝法检测K组分对培养的人脐静脉内皮细胞株ECV304的生长抑制情况,结晶紫法测定K组分对ECV304细胞的黏附抑制作用,鸡胚尿囊实验观察K组分对血管生成的影响。结果 K组分对人脐静脉内皮细胞株ECV304的增殖有抑制作用并呈明显的时-效及量-效关系,能够抑制ECV304细胞与纤维连接蛋白的黏附和鸡胚尿囊血管生成。结论广西尖吻蝮蛇毒K组分对血管生成有抑制作用。Objective To study the anti-angiogenesis effect of fraction K from Guangxi Agkitrodon Acutus venom. Methods The inhibitory effects of fraction K against endothelial cell line ECV304 in vitro was measured by MTT,adhesion of ECV304 cells to fibronectin（FN）cell by fraction K was observed.The anti-angiogenesis effect in vivo was evaluated by chick embryo chorioallantoic membrane（CAM）assay. Results The proliferation of endothelial cell line ECV304 was inhibited significantly by fraction K in a time and dose dependent manner.Fraction K also inhibited endothelial cell adhesion to FN and tubule formation in CAM. Conclusion Fraction K has an anti-angiogenesis activity.

关 键 词：尖吻蝮蛇毒 小肽 血管生成 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]