过表达SATB1对人原发性肝癌细胞HepG2增殖的促进作用  

Over-expession of SATB1 Promotes Hepatoma Cell HepG2 Proliferation

作　　者：罗敏[1] 涂炜[1] 刘永健[2] 邓欢[1] 周珍珍[1] 朱倩[1] 林海华[1] 夏丽敏[1] 晏维[1] 田德安[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,武汉430030 [2]华中科技大学同济医学院附属普爱医院内分泌科,武汉430033

出　　处：《医药导报》2011年第2期196-199,共4页Herald of Medicine

摘　　要：目的研究核基质结合蛋白SATB1对人原发性肝癌HepG2细胞增殖的影响。方法脂质体介导pEGFP1-SATB1真核表达全长基因质粒转染人原发性肝癌HepG2细胞,分为实验组(pEGFP1-SATB1)、对照组(pEGFP1空载体)及空白对照组。RT-PCR、Western blot检测转染后SATB1mRNA和蛋白表达变化情况,噻唑蓝(MTT)法、流式细胞仪检测SATB1全长基因转染对人原发性肝癌HepG2细胞生长、增殖的影响。结果与空白对照组及对照组比较,转染pEGFP1-SATB1后,HepG2中SATB1mRNA和蛋白表达明显增加,细胞生长曲线检测结果表明转导入pEGFP1-SATB1细胞增殖明显加快,细胞数明显增多;流式细胞仪检测结果表明,细胞周期中G0/G1期细胞明显减少,S、G2/M期细胞明显增多。结论 SATB1能明显促进HepG2细胞增殖,其机制可能与参与调控细胞周期各期DNA复制、转录有关。Objective To detect the effect of SATB1 on cell proliferation of hepatoma cell HepG2. Methods The HepG2 cells were over-expessed SATB1 by being transfected with plasmids encoding for whole length of SATB1 gene,the mRNA and protein levels were measured by semi-quantitative RT-PCR and Western blot analysis.The cell proliferation was determined by MTT assay,cell cycle distribution was detected by flow cytometry. Results After SATB1 over-expressing,compared with the blank and control groups,SATB1mRNA and protein were increased significantly in HepG2,and cell growth was promoted,cell number in G0/G1 phase was decreased and that in S and G2/M phase was increased. Conclusion The results showed that SATB1 over-expression promoted cell proliferation in HepG2 cell lines,and the mechanism of which may be associated with promoting DNA replication and transcription in cell phase.

关 键 词：SATB1 肝癌 原发性 细胞增殖 

分 类 号：R965[医药卫生—药理学]