苯唑西林与甲基紫的退色反应及应用  

Color fading reaction of oxacillin with methyl violet and its applications

作　　者：黄建琼[1] 江虹[1] 张华[1] 许正发[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《化学试剂》2011年第2期142-144,共3页Chemical Reagents

基　　金：重庆市教委科技基金资助项目(KJ101315);长江师范学院科研资助项目(2009xky008)

摘　　要：在pH8.6-9.9的条件下，苯唑西林（OXA）与甲基紫（MV）发生反应生成紫色离子缔合物，产生明显的退色现象，其最大和次大负吸收波长分别位于529和606nm，线性范围均为0-4．1μg／mL，表观摩尔吸光系数（ε）分别为2．63×10^4L·mo^-l ·cm^1（λ=529nm）和2．28×10^4L·mol^-1·cm^-1（λ=606nm），苯唑西林在一定浓度范围内服从比尔定律，据此建立了测定苯唑西林的退色分光光度法。用于市售苯唑西林药物中苯唑西林的测定，结果满意。At a pH value of 8.6～9.9,oxacillin(OXA) can react upon methyl violet(MV) to form purple ion association complexes and cause obvious fading.The maximum negative absorption wavelength and sub-major negative absorption wavelength of the system were 529 nm and 606 nm,respectively,and their linear range was 0～4.1 μg/mL and apparent molar absorptivity(ε) was 2.63×104 L·mol-1·cm-1(529 nm)and 2.28×104 L·mol-1·cm-1(606 nm),respectively.Oxacillin obeys the Beer′s law in a definite concentration range.A novel washed-out spectrophotometric method for the determination of oxacillin was thereby developed.This method has been applied to the determination of oxacillin content in the commercially available oxacillin medicament with satisfactory results.

关 键 词：苯唑西林 甲基紫 退色反应 分光光度法 

分 类 号：O657.3[理学—分析化学]