微流控芯片测定盐酸普鲁卡因注射液中盐酸普鲁卡因  

Determination of Procaine Hydrochloride in Procaine Hydrochloride Injection by Micro Total Analysis System

作　　者：蔡自由[1] 李永冲[1] 叶斌[1] 陈缵光[2] 

机构地区：[1]广东食品药品职业学院,广东广州510520 [2]中山大学药学院,广东广州510089

出　　处：《化学世界》2011年第1期17-19,29,35,共5页Chemical World

基　　金：国家自然科学基金资助项目(20575080;20727006)

摘　　要：建立了微流控芯片非接触电导检测法快速检测盐酸普鲁卡因注射液中盐酸普鲁卡因含量的方法。优化了缓冲溶液种类、浓度和配比,添加剂种类和浓度,分离电压及进样时间等电泳条件。结果表明,选择1 mmol/L HAc+3 mmol/L NaAc(pH4.5)为缓冲溶液,0.08 mmol/L SDS为添加剂,2.20 kV为分离电压,10 s为进样时间,盐酸普鲁卡因在2 min内可实现较好的分离和检测。盐酸普鲁卡因的线性范围为20～250μg/mL(r2=0.998),检出限为5.0μg/mL(S/N=3),RSD为1.8%,加标回收率为98.4%～99.0%。A new rapid method was established for the determination of the active component in procaine hydrochloride injection by micro total analysis system with contactless conductivity detection.The electrophoretic parameters,such as the variety and concentration of buffer solution and additive,separation voltage and injection time and etc,were optimized.Procaine hydrochloride（PH）was separated and detected within 2 min in the buffer of 1 mmol / L HAc ＋ 3 mmol / L NaAc（pH 4.5） with 0.08 mmol / L SDS at the separation voltage of 2.20 kV and injection for 10s.The linear concentration of PH ranged from 20 μg / mL to 250 μg / mL（r^2= 0.998）,and the limit of detection（LOD） reached 5.0 μg / mL（S/N=3）.The RSD was 1.8% and the recoveries ranged from 98.4% to 99.0%.

关 键 词：微流控芯片 非接触电导检测 盐酸普鲁卡因 

分 类 号：TQ462.8[化学工程—制药化工]