加味小承气颗粒剂对小鼠腹腔巨噬细胞合成白三烯B_4及对细胞内游离钙浓度的影响  

Effects of XiaoChengQi Granules on Leukotriene B 4 and (Ca 2+ )i of Peritoneal Macrophages in Mice

作　　者：王淑芬[1,2] 肖向茜[1,2] 翁福海 

机构地区：[1]天津干部疗养院 [2]天津医科大学

出　　处：《中国中西医结合外科杂志》1999年第4期210-213,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

摘　　要：目的：研究加味小承气（ＸｉａｏＣｈｅｎｇＱｉ，ＸＣＱ）颗粒剂治疗腹腔炎症机理与小（大）鼠腹腔巨噬细胞（ＭΦ）合成释放白三烯Ｂ４以及对细胞内游离钙浓度的影响。方法：应用高效液相色谱分析ＬＴＢ４，应用Ｆｕｒａ－２／ＡＭ及荧光分光光度计测定细胞内钙离子浓度。结果：不同浓度的ＸＣＱ颗粒剂（０１５～１０ｇ／Ｌ）明显抑制大鼠（ＭΦ）合成释放白三烯Ｂ４并抑制小鼠细胞内钙离子释放（静止期细胞内钙离子浓度为６３４５±１２４４ｎｍｏｌ／Ｌ，与对照组（１４８３９±１６６２ｎｍｏｌ／Ｌ）比较有显著性差别，在细胞外钙浓度增高条件下，ＸＣＱ尚促使外钙内流。结论：小承气颗粒剂抗炎机理与抑制小（大）鼠（ＭΦ）合成释放白三烯Ｂ４及调节细胞内外钙离子浓度有关。Objective:To further study the relationship between leukotriene B 4 and Ca 2+ i of peritoneal macrophages inhibited by XCQ granules.Methods:Using Rp HPLC to check LTB 4 and measuring concentration of intracellular calcium Ca 2+ i of peritoneal macrophages by spectrofluorophotometer.Results:XCQ inhibited both synthesis of LTB 4 and intracellular calcium release of macrophages.With appropriated dosage,XCQ could promote influx of extracellular calcium.Conclusion:XCQ franules inhibited the synthesis of LTB 4 significantly and reduced intracellular calcium release,also accelerate influx of extracellular calcium into cell.The mechanism might be its anti inflammatory effect.

关 键 词：小承气颗粒剂 白三烯B4 巨噬细胞 钙 

分 类 号：R285.5[医药卫生—中药学]