HIV/AIDS患者精子中病毒核酸的检测  

Detection of the HIV-1 nucleic acid in spermatozoa of HIV/AIDS patients

作　　者：王典[1] 李练兵 侯志伟 谢庆东[1] 康祥锦[1] 黄天华[1] 

机构地区：[1]汕头大学医学院生殖医学研究中心,广东汕头515041 [2]重庆市出生缺陷与生殖健康市级重点实验室,400020

出　　处：《广东医学》2011年第2期175-177,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30800940)

摘　　要：目的检测HIV/AIDS患者精子中HIV核酸。方法制备33例HIV/AIDS患者精子样本,采用荧光原位杂交(FISH),以生物素标记的HIVgag及HIVpol作为双探针对这些患者精子进行HIV核酸检测。结果在9例(27.3%)患者中检测到阳性荧光信号。结论 HIV/AIDS患者精子存有HIV核酸;FISH是一种检测HIV/AIDS患者精子病毒核酸灵敏而可靠的方法。Objective To detect the presence of HIV-1 nucleic acid in spermatozoa of HIV/AIDS patients.Methods Spermatozoon samples of 33 HIV/AIDS patients were prepared.Fluorescence in situ hybridization（FISH）,in which biotinylated HIV gag and HIV pol were used as probes,was performed to detect HIV nucleic acid in spermatozoa.Results HIV nucleic acid was detected in spermatozoa of 9 patients（27.3%）.Conclusion These results further confirm the presence of HIV nucleic acid in sperm of HIV/AIDS patients and indicates that FISH is a sensitive and reliable method to detect the nucleic acid in sperm.

关 键 词：HIV/AIDS患者 精子 HIV核酸 荧光原位杂交 

分 类 号：R512.91[医药卫生—内科学]