槲寄生药材的高效液相色谱指纹图谱研究  

Investigation on the High Performance Liquid Chromatography Fingerprints of Viscum coloratum (Komar) Nakai

作　　者：袁庆芳[1] 赵春梅[2] 张忠鹏[1] 杨官娥[1] 

机构地区：[1]山西医科大学药学院中药学教研室,山西太原030001 [2]东北林业大学帽儿山教学区,黑龙江哈尔滨150611

出　　处：《分析科学学报》2011年第1期16-20,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.30672621);山西省自然科学基金(No.2006011199);山西医科大学博士启动基金(03200811)

摘　　要：采用Diamonsil C18(2)色谱柱,乙腈-0.1%磷酸梯度洗脱,测定了15批不同产地的槲寄生样品,建立其指纹图谱共有模式;采用"中药色谱指纹图谱相似度评价系统"软件进行数据处理,聚类分析,建立槲寄生药材高效液相色谱指纹图谱。结果表明:以高圣草素-7-O-β-D-芹菜糖基(1→2)-β-D-葡萄糖苷为参照物峰,确定18个共有峰,相似度>0.7,建立了槲寄生药材的指纹图谱,可用于不同产地槲寄生药材的指纹图谱测定和质量评价。Reversed phase-high performance liquid chromatography（RP-HPLC） was used to investigate the fingerprints of Viscum coloratum.15 batches of Viscum coloratum samples from different areas were analyzed by RP-HPLC with acetonitrile-phosphoric acid solution（0.1%） as the mobile phase and gradient elution on a Diamonsil C18（2） column was processed.＂Similarity Evaluation System for Chromatographic Fingerprint of TCM＂ and clustering method were used for data analysis and fingerprint investigation.18 chromatographic peaks were identified as the specific fingerprint with naringenin 7-O-apiofuranosyl（1→2）-β-D-glucopyranoside as the reference substance,and the similarities were all above 0.7.Based on the established fingerprint chromatogram of Viseum coloratum,identification and quality evaluation of Viseum coloratum from different areas would be processed.

关 键 词：槲寄生 指纹图谱 反相高效液相色谱法 

分 类 号：O657.72[理学—分析化学]