毛细管电泳法手性拆分达卢生坦中间体2-羟基-3-甲氧基-3,3-二苯基丙酸  

Enantiomeric Separation of 2-Hydroxy-3-Methoxy-3,3-Diphenylpropionic Acid by High Performance Capillary Electrophoresis

作　　者：谷建敏[1,2] 付炎[3] 孟静[2] 张兰桐[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北石家庄050017 [2]河北医科大学药学院药物化学教研室,河北石家庄050017 [3]河北医科大学药学院实验中心,河北石家庄050017

出　　处：《分析科学学报》2011年第1期45-48,共4页Journal of Analytical Science

基　　金：河北省教育厅自然科学研究指导项目(Z2008124);河北省自然科学基金资助项目(2010000494)

摘　　要：建立了毛细管电泳法拆分2-羟基-3-甲氧基-3,3-二苯基丙酸的方法。考察了背景电解质的pH值和浓度、手性选择剂的种类及浓度、有机改性剂种类及浓度对分离的影响,并对分离条件进行了优化。实验结果表明,在60 mmol/L磷酸氢二钠(pH=9.10)为运行缓冲溶液,含35.7 mmol/L羟丙基-β-环糊精和5%甲醇的体系中,对映体达到基线分离。S型及R型对映异构体均在5～160μg/mL浓度范围内线性关系良好,平均加标回收率为100.3%,检出限为1.01μg/mL。The enantiomers of 2-hydroxy-3-methoxy-3,3-diphenylpropionic acid were successfully separated by high performance capillary electrophoresis using HP-β-CD as a chiral selector.Effects of the pH and concentration of buffer,concentration of HP-β-CD,type and concentration of organic modifier on the resolution of enantiomers were investigated.Under the optimized conditions pH=9.10 60 mmol/L of phosphate buffer solution,35.7 mmol/L HP-β-CD and 5% CH3OH,the enantiomers of 2-hydroxy-3-methoxy-3,3-diphenylpropionic acid were separated baseline.Calibration curve was linear over the range of 5～160 μg/mL,and the limit of detection was 1.01 μg/mL for target analyte.

关 键 词：达卢生坦 毛细管电泳 手性拆分 羟丙基-Β-环糊精 

分 类 号：O657.8[理学—分析化学]