大鼠胆汁中人参皂苷和淫羊藿黄酮苷的HPLC-UV/ELSD法测定  

Determination of Ginsenosides and Epimedium Flavonoids in Rats Bile by HPLC-UV/ELSD

作　　者：刘敏彦[1] 张兰桐[1] 赵韶华[2] 王海荣[2] 王胜[2] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北石家庄050017 [2]石家庄以岭药业股份有限公司,河北石家庄050035

出　　处：《中国医药工业杂志》2011年第2期122-124,148,共4页Chinese Journal of Pharmaceuticals

基　　金：国家科技重大专项课题(2009ZX09313-003;2009ZX09102-140)

摘　　要：建立了HPLC-紫外检测器串联蒸发光散射检测器同时测定大鼠尾静脉注射肌萎灵冻干粉后胆汁中的人参皂苷Rg1、Re,淫羊藿苷和朝藿定A、B、C。采用C_(18)色谱柱,乙腈-水为流动相,梯度洗脱,检测波长220 nm;蒸发光散射检测器检测:漂移管温度80℃,气体压力25 psi。人参皂苷Rg1、Re,淫羊藿苷和朝藿定A、B、C分别在11.64～698.1、8.59～515.4、2.70～323.4、1.53～183.6、1.04～499.2和1.37～878.4μg/ml浓度范围内线性关系良好,提取回收率为89.53～99.54%,日内和日间RSD均小于9.28%,相对误差-9.85%～5.10%。An HPLC-UV/ELSD method was established for the simultaneous determination of ginsenosides （Rg_1,Re）,icariin and epimedins（A,B,C） in rat bile after intravenous administration of Jiweiling lyophilized powder. A C_（18） column was used with acetonitrile-water as mobile phase by gradient elution at the detection wavelength of 220 nm.An evaporative light scattering detector（ELSD） was used at drift tube temperature of 80℃and gas pressure of 25 psi.The calibration curves of ginsenosides（Rg_1,Re）,icariin and epimedins（A,B,C） were linear in the concentration ranges of 11.64-698.1,8.59-515.4,2.70-323.4,1.53-183.6,1.04-499.2 and 1.37-878.4μg/ml,respectively.The average extraction recoveries were 89.53%-99.54%.The intra- and inter-day RSDs were less than 9.28%and the relative errors were within the range of-9.85%-5.10%.

关 键 词：肌萎灵冻干粉 人参皂苷 淫羊藿黄酮苷 高效液相色谱-紫外检测器串联蒸发光散射检测器 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]