聚(α,β-L-天冬氨酸丁酯)的合成及水解降解行为研究  

SYNTHESIS AND HYDROLYTIC DEGRADATION OF POLY(α,β-BUTOXY-L-ASPARTATE)

作　　者：王勇[1,2] 王亦农[1] 伍国琳[1] 范云鸽[1] 马建标[3] 

机构地区：[1]功能高分子材料教育部重点实验室,南开大学高分子研究所,天津300071 [2]中国协和医科大学,中国医学科学院生物医学工程研究所,天津300192 [3]天津理工大学化工学院,天津300191

出　　处：《离子交换与吸附》2011年第1期10-17,共8页Ion Exchange and Adsorption

基　　金：天津市自然科学基金(09JCYBJC03400,10JCYBJC26800);973计划前期研究专项(2009CB626612);高等学校博士学科点专项科研基金资助课题;中央高校基本科研业务费专项资金资助

摘　　要：以L-天冬氨酸为原料,采用热缩聚法合成聚琥珀酰亚胺。选用醇钠作为亲核试剂,开环反应得到聚(α,β-烷氧基-L-天冬氨酸)。该聚合物部分水解制备两亲性聚天冬氨酸(PAsp-Na/PAsp-R)。以聚(α,β-L-天冬氨酸丁酯)为例,利用UV、1H NMR等方法研究聚合物的水解降解行为。通过1H NMR中侧链酯基信号的消失和主链游离氨基在紫外光谱中吸光度的变化,证明水解降解过程包括侧链酯键的水解和主链酰胺键的适度裂解两部分,水解过程受环境温度和缓冲溶液pH的共同影响。水解速率具有温度响应性,环境温度越高水解速率越快(37℃时的水解速率>25℃时的水解速率)。缓冲溶液pH对聚合物降解速率的影响顺序为:pH12>pH4>pH7。Novel poly（α,β-alkoxy-L-aspartates） were successfully synthesized by nucleophilic substitution in polysuccinimide which was the thermal polycondensation product of L-aspartic acid.Amphiphilic polymer PAsp-Na/PAsp-R could be obtained by partially hydrolysis of poly（α,β-alkoxy-L-aspartates）.Hydrolysis process of poly（α,β-butoxy-L-aspartates）（PAsp-B） was studied by UV spectroscopy and 1H NMR.The results showed that the degradation of PAsp-B included the rapid elimination of butyl side chains and the moderate cleavage of peptide linkages in backbone as well as that the hydrolytic properties of PAsp-B were controlled by hydrolytic environments such as temperature and pH value.The hydrolysis rate of PAsp-B in aqueous solutions decreased in the order of pH 12pH 4pH 7 and increased with increasing the environmental temperature（for example,the hydrolysis rate of PAsp-B was higher at 37℃ than that at 25℃）.

关 键 词：聚天冬氨酸 降解 两亲性 环境敏感生物高分子 

分 类 号：O631[理学—高分子化学]