同步辐射圆二色谱分析纳米颗粒对细胞DNA稳定性的影响  

Effects of nanoparticles on stability of cell DNA studied by SRCD

作　　者：张铭倚[1] 王鹏[2] 邢更妹[1] 张钧[2] 

机构地区：[1]中国科学院高能物理研究所,北京100049 [2]北京理工大学生命学院,北京100081

出　　处：《核技术》2011年第2期134-138,共5页Nuclear Techniques

基　　金：国家自然科学基金(10875135);科技部国家重点基础研究发展计划项目(2009CB930204)资助

摘　　要：选用30 nm和50 nm羧基化聚苯乙烯微球(COOH-PBs)作用于RAW264.7巨噬细胞,用真空紫外圆二色光谱(SRCD)分析细胞DNA二级结构的可能变化,并用彗星电泳验证此法的可行性和优越性。研究结果显示,两种颗粒都致使DNA螺旋度降低及单链程度增加,30 nm COOH-PBs造成的损伤甚于50 nm,表明纳米颗粒影响DNA的稳定性有粒径依赖性。SRCD为深入研究纳米颗粒对细胞DNA稳定性的影响及其可能原因提供了一个可行的研究方法和途径。To investigate influence of different-sized nanoparticles on stability of cell DNA, RAW264.7 strains were incubate with 30 nm and 50 nm COOH-labeled polystyrene beads （COOH-PBs）. Synchrotron radiation circular dichroism spectroscopy （gRCD） was used to analyze possible transformation of the secondary structure of DNA, which was further confirmed by means of comet assay. The CD spectra indicated that both particles could reduce helicity of the DNA and increase the degree of DNA single strands. The 30 nm-PBs treatment did more damage than the 50 nm-PBs treatment. This suggests a size-dependent influence. It is demonstrated that SRCD provides a reliable method to investigate the stability of cell DNA influenced by nanoparticles.

关 键 词：DNA 纳米颗粒 SRCD 

分 类 号：Q945.79[生物学—植物学]