二阶导数线性扫描极谱法测定秋茄叶中总黄酮含量  

Determination of total flavones in Kandelia candel leaves by second derivative linear-sweep polarography

作　　者：范润珍[1] 林树群 杨素萍[3] 宋文东[1] 

机构地区：[1]广东海洋大学理学院,广东湛江524025 [2]汕头市美宝制药有限公司,广东汕头515041 [3]河北北方学院基础农学部,河北张家口075000

出　　处：《台湾海峡》2011年第1期138-141,共4页Journal of Oceanography In Taiwan Strait

基　　金：广东省自然科学基金资助项目(7010100);湛江市科技招标资助项目(0709191)

摘　　要：探讨了秋茄叶中黄酮类物质的极谱特性,并进行了含量测定.结果表明,在pH值为9.18的硼砂底液中,以芦丁作对照品,秋茄叶中黄酮类化合物在峰电位Ep为-1 642 mV(vs.SCE)处产生一灵敏的二阶导数极谱波,在芦丁含量不超过1.4 mg/dm3范围内,该极谱波的峰电流(Ip)与芦丁的量之间呈良好的线性关系,据此建立了红树秋茄叶中总黄酮含量的极谱定量测定方法.该方法的检出限为0.02 mg/dm3.用此法测得红树秋茄叶中总黄酮含量为2.04%,回收率为96.4%～100.3%,相对标准偏差为3.19%,此方法灵敏、准确、干扰少.Polarographic characteristics of flavones from the leaves of Kandelia candel were studied and the contents of the flavones were determined.The results showed that a sensitive second derivative polarographic wave was produced at the potential of Ep-1 642 mV（vs.SCE） in a solution of borax with a pH of 9.18,in which rutin was used as a reference.The current peak（Ip） of the polarographic wave showed a good linear proportional relationship to rutin,with the rutin concentration being less than 1.4 mg/dm3.Therefore,a polarographic method for the quantitative determination of flavones in the leaves of Kandelia candel was established.The detection limit of this method was 0.02 mg/dm3.Overall,2.04% of the flavones in the Kandelia candel leaves were determined by this method with recoveries in the range of 96.4%～100.3% and an relative standard deviation of 3.19%.This method was sensitive,accurate and subject to little interference.

关 键 词：海洋化学 秋茄 黄酮 二阶导数线性扫描极谱法 

分 类 号：P734[天文地球—海洋化学]