阿尔茨海默病患者体内端粒甲基化分析  

Methylation of telomeres in patients with Alzheimer's disease

作　　者：管静芝[1] 管维平[2] 朱文华[1] 

机构地区：[1]解放军第309医院肿瘤科,北京100091 [2]解放军总医院南楼神经内科,北京100853

出　　处：《军医进修学院学报》2011年第2期120-121,150,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：解放军第309医院内科研启动基金(#09QD02)

摘　　要：目的探讨阿尔茨海默病(Alzheimers′Disease,AD)患者体内端粒甲基化变化规律。方法39名AD患者,57名年龄相关的正常对照。用限制性内切酶(Moraxella Strain Producing the Enzyme I,MspI)与Haemophilus Parainfluenzae II(HpaII)评估外周血的端粒甲基化分布。结果AD患者组MspI和HpaII内切酶的端粒平均长度与对照组相比差异均无统计学意义,但都与年龄呈负相关。在最长端粒(>9.4kb)和最短端粒(<4.4kb)长度区域,AD组端粒的甲基化程度明显高于对照组(0.28±0.16 vs 0.22±0.12,P=0.021;0.24±0.11 vs 0.46±0.15,P<0.001)。结论提示了AD患者体内加速的年龄相关的老化特性。Objective To study the changing rules of telomere methylation in patients with Alzheimer＇s disease（AD）.Methods Distribution of telomere methylation in peripheral blood of 39 patients with AD and 57 healthy controls was detected with restriction endonucleases MspI and HpaII.Results The average length of telomeres detected with MspI and HpaII in patients with AD and in healthy controls was not significantly different but negatively related with the age of patients with AD.The methylation extent of the longest telomere（9.4kb） and the shortest telomere（4.4kb） was significantly higher in patients with AD than in healthy controls（0.28±0.16 vs 0.22±0.12,P=0.021,0.24±0.11 vs 0.46±0.15,P0.001）.Conclusion Accelerated methylation of telomeres in patients with AD is their aging-related features.

关 键 词：阿尔茨海默病 端粒 甲基化 

分 类 号：R34[医药卫生—基础医学]