两种常用转染试剂转染siRNA至HL-60细胞转染效率的比较  

Efficiency comparison of two transfection reagents on transfecting siRNA to HL-60 cells

作　　者：王巍[1] 张晓希[2] 刘新光[3] 

机构地区：[1]广东医学院临床血液检验学教研室,东莞523808 [2]广东医学院中国人民解放军第四二二医院,广东湛江524023 [3]广东医学院检验医学研究所,东莞523808

出　　处：《重庆医学》2011年第4期313-314,318,F0002,共4页Chongqing medicine

基　　金：广东省教育厅自然科学研究项目(Z03042)

摘　　要：目的比较两种常用转染试剂转染小干扰RNA(siRNA)至悬浮细胞的转染效率及对细胞毒性的影响。方法以羧基荧光素(FAM)标记的siRNA为报告基因,以lipofectamine 2000和siPORT NeoFX为转染试剂,用流式细胞仪检测转染效率,倒置显微镜观察细胞形态,MTT法检测细胞存活率。结果 siRNA>100 nmol/L时,lipofectamine 2000的转染效率高于siPORT NeoFX(P<0.05);si RNA<100 nmol/L时,前者低于后者(P<0.05)。siRNA终浓度及转染试剂用量相同时,lipofectamine 2000组HL-60细胞存活率与SiPORT NeoFX组比较,差异无统计学意义(P>0.05)。结论在使用高浓度si RNA时,lipofectamine2000对HL-60细胞有较高的转染效率和较小细胞毒性。Objective To compare the transfection efficiency and cytotoxicity between two cationic transfection reagents.Methods Small interfering RNA（siRNA） marked with FAM as a report gene,lipofectamine 2000 and siPORT NeoFX were used as the transfection reagents.Flow cytometer,microscope and MTT assay were used to detect transfection efficiency and cytotoxicity.Results The transfection efficiency of lipofectamine 2000 was higher than siPORT NeoFX when siRNA was over 100 nmol/L（P0.05）,otherwise with siRNA under 100 nmol/L（P0.05）.At the same siRNA concentration and transfection reagent volume,there was no significant difference of vial cells ratios between two groups（P0.05）.Conclusion lipofectamine 2000 is an effective and safe transfection reagent to HL-60 cells when siRNA is over 100 nmol/L.

关 键 词：脂质体 转染 RNA 小分子干扰 

分 类 号：R346[医药卫生—基础医学]