新型骨科材料SCPP对成骨细胞促血管化生长因子表达影响的研究  

Study of the Influence of SCPP on Angiogenic Growth Factors Expression of Osteoblasts

作　　者：余喜讯[1,2] 顾志鹏[1] 任大伟[1,2] 刘菲[1] 冯婷[3] 张小华[1] 万昌秀[1] 

机构地区：[1]四川大学高分子科学与工程学院,四川成都610065 [2]四川大学苏州研究院,江苏苏州215123 [3]四川大学华西第二医院港大联合中心,四川成都610041

出　　处：《四川大学学报（工程科学版）》2011年第1期219-225,共7页Journal of Sichuan University (Engineering Science Edition)

基　　金：国家自然科学基金资助项目(30870616);江苏省自然科学基金资助项目(BK2008152);四川省应用基础研究资助项目(07JY029-075)

摘　　要：在制备聚磷酸钙过程中掺入锶获得掺锶量为(1%、2%、5%、8%、10%)的新型骨科材料SCPP。研究SCPP对成骨细胞行为及其血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的影响,为阐明其促血管化机理提供实验依据。以成骨细胞与支架材料复合培养,采用XRD,MTT法,ALP活性检测,SEM观察,RT-PCR和ELISA技术等对其进行表征。结果表明,SCPP能够促进成骨细胞增殖,提高其ALP活性,上调成骨细胞VEGF和bFGF的mRNA和蛋白表达,与对照组CPP均有显著性差异(P<0.05),以8%SCPP最为明显。SCPP具有"促血管化作用",是一个很有前景的骨组织工程支架或骨修复材料。Strontium-doped calcium polyphosphate（SCPP） with the content of 0,1,2,5,8,10 mol% Sr^2＋,respectively,were prepared as a novel bone material.The influence of SCPP on cellular behavior and angiogenic factors which were vascular endothelial growth factor（VEGF） and basic fibroblastic growth factor（bFGF） of osteoblasts was investigated,which provided the experimental basis for elucidating mechanism of SCPP promoting vascularization.Osteoblasts were cultured with SCPP scaffolds,XRD,MTT assay,quantitative detection of ALP activity,scanning electron microscopy（SEM） observation,Reverse Transcription-Polymerase Chain Raction（RT-PCR） analysis and Enzyme-linked Immunosorbent Assay（ELISA） were used to investigate.The results showed that,compared with CPP（P0.05）,the control,cellular proliferation,ALP activity as well as the mRNA and protein expression of the angiogenic factors（VEGF and bFGF） of the SCPP group were significantly promoted,and 8% SCPP showed the highest expression.

关 键 词：掺锶聚磷酸钙 血管化 血管内皮生长因子 碱性成纤维细胞生长因子 

分 类 号：R318[医药卫生—生物医学工程] TQ174[医药卫生—基础医学]