大肠癌患者外周血检测P^53表达及其临床意义  

作　　者：席纪江 

机构地区：[1]江苏省徐州市第一人民医院普通外科,221002

出　　处：《浙江临床医学》2011年第2期126-128,共3页Zhejiang Clinical Medical Journal

摘　　要：目的探讨大肠癌患者外周血检测P^53的表达情况及其临床意义。方法采用流式细胞术，于术前1d、术后1d及术后7d分别对109例大肠癌患者用外周血检测P^53表达，实验数据用SPSS统计软件处理。结果术前1d检测，P^53有异常表达，手术后1d和第7天检测，P^53表达阳性率较术前1d检测的阳性表达率增加，差异有统计学意义（P〈0．05）。结论血循环中肿瘤细胞的存在是大肠癌的一种常见早期现象，外周血中可检出大肠癌细胞生物学标志物矿。突变表达；同时，手术刺激可增加肿瘤细胞向血循环中释放。Objective To study the clinical value of the expression of P53 gene in peripheral blood in the patients with colorectal cancer. Methods Flow eytometry （FCM） was used to detect p53 gene mutation expression in peripheral blood tumour cells of 109 patients with colorectal cancer. Experimental data were analyzed by SPSS （ v. 11.0 ） software. Results Abnormal expresion of p53 gene circulating turnout cells in the peripheral blood in the patients with colorectal cancer was found before operation. Positive rate of p53 gene in peripheral blood after operation was siugnificently higher than that before operation （P 〈 0. 05 ）. Conclusion Detection the expression of P53 gene might be helpful to the early diagnosis of coloreetal cancer. Molecular detection in peripheral blood at surgery suggests that hematogenie tumor cell dissemination is a common and early event, the mutation p53 expression in cancer cells of peripheral blood may be detected. Surgical manipulation enhances this release of tumor ceils into the circulation.

关 键 词：循环肿瘤细胞 大肠癌 P^53基因 外科手术 

分 类 号：R135.1[医药卫生—劳动卫生]