半制备高效液相色谱法分离纯化川木通中齐墩果酸  

Isolation and purification of oleanolic acid in caulis clematidis armandii by semi-preparative HPLC

作　　者：杨胜丹 余林春[2] 付大友[3] 

机构地区：[1]四川天正化工有限责任公司,四川成都610218 [2]四川省天然气化工研究院,四川成都610212 [3]四川理工学院材料与化学工程学院,四川自贡643000

出　　处：《应用化工》2011年第1期173-175,共3页Applied Chemical Industry

摘　　要：采用半制备高效液相色谱法对川木通中有效成分齐墩果酸进行分离纯化,纯化齐墩果酸的制备色谱条件:半制备型色谱柱ZORBAX SB-C18(9.4 mm×150 mm,5μm),流动相乙腈-水=87∶13,流速3 mL/min,柱温室温,检测器VWD检测器,波长210 nm,进样量20μL。对制备出的馏分进行定性分析。该法具有纯化效果好、操作高效、快速的特点。Semi-preparative HPLC was used to isolate and purify the active constituent of oleanolic acid from caulis clematidis armandii.The preparative chromatography conditions for the preparation of oleanolic acid were established： semi-preparative column of ZORBAX SB-C18（9.4 mm×150 mm,5 μm）,and mobile phase was acetonitrile-water（87 ∶13）,flow rate was 3 mL/min,wavelength was 210 nm,column temperature was room temperature,injection volume was 20 μL.The prepared fractions were analysized qualitatively.This method has the characteristics of purify effective,efficient operation,and quickly to purify oleanolic acid in caulis clematidis armandii.

关 键 词：川木通 齐墩果酸 分离 纯化 半制备高效液相色谱法 

分 类 号：O657.72[理学—分析化学]