产铁载体PGPR菌筛选及其对病原菌的拮抗作用  

Screening of siderophore-producing PGPR bacteria and their antagonism against the pathogens

作　　者：荣良燕[1,2] 姚拓[1,2] 赵桂琴[1,2] 柴强[3] 席琳乔[4] 王小利 

机构地区：[1]甘肃农业大学草业学院,兰州730070 [2]草业生态系统教育部重点实验室,兰州730070 [3]甘肃农业大学农学院,兰州730070 [4]塔里木大学动物科技学院,阿拉尔843300 [5]贵州省草业研究所,独山558200

出　　处：《植物保护》2011年第1期59-64,共6页Plant Protection

基　　金：国家科技支撑计划基金(2007BAD89B17);国家自然科学基金(30960265);农业部燕麦产业体系基金;贵州省科技攻关项目(黔科合NY字[2009]3067)

摘　　要：采用改进的CAS定性、定量方法,从16株PGPR菌株中初步筛选出具有抗病原真菌作用的菌株,再利用平板对峙法将筛选出的5株PGPR菌与3种病原真菌进行拮抗试验。结果表明,LHS11对黄瓜枯萎病菌(Fusarium oxysporum f.sp.cucumerinum),西瓜枯萎病菌(Fusarium oxysporum f.sp.niveum)的抑制效果最好,抑菌率达到80%以上;其次是191对黄瓜枯萎病菌,抑菌率为74%,LHS11、191对立枯丝核菌(Rhizoctonia solani)的抑制效果相近,抑菌率分别为64%和65%。191和LHS11是抑菌效果较好的生防PGPR菌株,具有较好的应用潜力。By adopting improved CAS methods, the strains antagonistic against plant pathogens were firstly screened from the 16 PGPR strains, and the antagonistic experiments were then conducted by using 5 PGPR strains to 3 types of plant pathogens using plate cultivation. The results showed that LHSll had the best inhibitory effects on Fusarium oxysporum f. sp. cucurnerinum and F. oxysporum f. sp. niveum, with the inhibition ratio over 80% ; the inhibition ratio of 191 against F. oxysporum f. sp. cucumerinum was 74%. The inhibitory effects of LHS11 and 191 on Rhizoctonia solani were similar （ 64% and 65%, respectively）. Both 191 and LHSll could be used as PGPR biocontrol agents with good inhibitory effects and high application potential.

关 键 词：产铁载体菌 CAS检测 拮抗作用 病原真菌 PGPR菌株 

分 类 号：Q93[生物学—微生物学]