侧孢芽孢杆菌2-Q-9菌株外泌抗菌肽的纯化与鉴定  

Purification and characterization of antimicrobial peptide secreted by Bacillus laterosporus 2-Q-9

作　　者：陈武[1] 彭曙光 周清明[1] 黎定军[4,5] 罗宽[4] 

机构地区：[1]湖南农业大学作物学博士后流动站,湖南长沙410128 [2]湖南省烟草公司烟叶处,湖南长沙410007 [3]中国烟草中南农业试验站,湖南长沙410128 [4]湖南农业大学中国烟草中南农业试验站,湖南长沙410128 [5]湖南广播电视大学,湖南长沙410004

出　　处：《湖南农业大学学报（自然科学版）》2011年第1期26-30,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省自然科学基金项目(BK0431);湖南省教育厅青年项目(03B012)

摘　　要：采用75%乙醇沉淀、阴离子交换色谱层析和阳离子交换色谱层析等方法,分离并纯化侧孢芽孢杆菌(Bacillus laterospours)2-Q-9菌株外泌抗菌肽BL2Q9。结果表明,BL2Q9的相对分子质量为7 800,等电点为10.2。Edman降解测序发现其N末端封闭,串联质谱测序结果表明其与细菌第Ⅳ类鞭毛合成蛋白pilQ的前体蛋白以及转录抑制子CodY蛋白的部分序列相似,但与已知抗菌肽的序列相似性较低。Bacillus laterosporus stranin 2？Q？9 secreted a high level of antimicrobial peptides when cells were grown in liquid NB media at 28 ℃ for 24 h.Purification was carried out by protein precipitation with 75% ethanol,anion-exchange chromatography and cation-exchange chromatography sequentially.The relative molecular weight and isoletric point of antimicrobial peptide BL2Q9 were 7 800 and 10.2 respectively.The sequencing results of MS/MS showed that the antimicrobial peptide was a new antimicrobial peptide（AMP） with low amino acid sequence similarity among the known AMPs in database.

关 键 词：侧孢芽孢杆菌 外泌抗菌肽 纯化与鉴定 质谱测序 

分 类 号：Q516[生物学—生物化学]