百合抗尖孢镰刀菌无性系的离体筛选  

In vitro selection for lily clonal lines with Fusarium oxysporum f.sp.lilii toxin

作　　者：丁丁[1,2,3] 吕长平[3] 王继华[1,2] 崔光芬[1,2] 吴学尉[1,2] 张艺萍[1,2] 

机构地区：[1]云南省农业科学院花卉研究所,云南昆明650205 [2]云南省花卉育种重点实验室,云南昆明650205 [3]湖南农业大学园艺园林学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2011年第1期34-38,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：云南省科技计划项目(2006NG14);国家科技支撑计划项目(2006BAD01A1803;2007BAD45B01);云南省社会发展科技计划项目(2009ZC140M)

摘　　要：以百合品种元帅(Acapulco)、卡萨布兰卡(Casablanca)的愈伤组织为试验材料,附加不同浓度的枯萎病菌毒素粗提液,用于抗尖孢镰刀菌无性系的离体筛选。结果表明,以体积分数75%的毒素培养基上培养10 d作为百合抗尖孢镰刀菌无性系筛选的选择压比较适宜,抗镰刀菌无性系的再生苗生长正常,叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)活性均比未经筛选的对照株高,经人工接种枯萎病菌鉴定,2个百合品种抗尖孢镰刀菌无性系的再生植株均表现中抗枯萎病。In order to obtain anti-Fusarium clones of lily,callus of Acapulco and Casablanca were employed as material for the seclection of anti-Fusarium clones by adding various concentrations of toxin in MS medium.The result showed that medium containing 75% of the toxin and 10 d culture can be used as selection pressure for screening lily anti-Fusarium clones and its regeneration plants of disease-resistance had healthy growth.It was found that activity of three enzymes,POD,PPO and SOD,in screened plants was higher than that of control and the screened plants were healthy.Lily anti-Fusarium clones showed a middle level of resistance to lily blight disease by identification of disease-resistance levels.

关 键 词：百合 尖孢镰刀菌 无性系 枯萎病 

分 类 号：S644.1[农业科学—蔬菜学]