聚丙烯酸的凝胶渗透色谱分离及相对分子质量测定  

Separation of Polyacrylic Acid and Determination of Its Relative Molecular Mass by Gel Permeation Chromatography

作　　者：杜小弟[1] 张安富[1] 雷家珩[1] 巫辉[1] 

机构地区：[1]武汉理工大学理学院化学系,湖北武汉430070

出　　处：《分析测试学报》2011年第2期167-170,175,共5页Journal of Instrumental Analysis

基　　金：交通部西部交通建设科技项目(2007319811130)

摘　　要：采用Sepax Nanofilm SEC-150色谱柱和自制窄分布聚丙烯酸标样，考察了不同无机盐及其离子强度、酸度等对不同相对分子质量的聚丙烯酸分离效果的影响。在此基础上确定了凝胶渗透色谱分离聚丙烯酸的最佳条件：以磷酸盐缓冲液（离子强度0．10mol／L，pH=6．9）为流动相，流速1．0mL／min，柱温25℃，试样的质量浓度为0．5～2．0g／L，进样量50μL。在优化实验条件下，可按分子大小实现对聚丙烯酸的分离。通过渐近校正法对自制聚丙烯酸标样的峰值相对分子质量定值，并对待测聚丙烯酸样品的相对分子质量及其分布进行测定。Using Sepax Nanofilm SEC-150 chromatographic column and self-made narrow distribution polyacrylic acid （PAA） standard sample, effects of different inorganic salts and their ionic strength as well as pH value on the separation of PAA were investigated. The results indicated that the different relative molecular masses of PAA with mass concentration of 0. 5 - 2.0 g/L could be separated by using ionic strength of 0. 10 mol/L and pH 6.9 phosphorous buffer as mobile phase, with a flow rate of 1.0 mL/min, column temperature of 25 ℃ and sample volume of 50 μL. Under the optimal chromatographic conditions, the relative molecular mass and its distribution of PAA sample were measured accurately by the asymptotic calibration method, and the difference between result of viscometric and GPC methods was less than 10% .

关 键 词：聚丙烯酸 相对分子质量 凝胶渗透色谱 非体积排除效应 

分 类 号：O657.7[理学—分析化学] TQ325.7[理学—化学]