西瓜中4种植物生长调节剂残留的分析方法研究  

Study on Method of Determination of Four Plant Growth Regulater Residues in Watermelons

作　　者：陆益民[1,2] 易国斌[1] 陈创彬[2] 蔡金亮[2] 杜彬仰[2] 陈如珠[2] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]仲恺农业工程学院化学化工学院,广东广州510225

出　　处：《分析测试学报》2011年第2期186-189,共4页Journal of Instrumental Analysis

基　　金：广东省科技计划资助项目(07001781);仲恺农业工程学院资助项目(G3072709)

摘　　要：建立了固相萃影高效液相色谱法测定西瓜中2，4-二氯苯氧乙酸（2，4-D）、赤霉素（GA3）、多效唑（PP333）、6-苄氨基嘌呤（6-BA）4种植物生长调节剂残留的方法。样品用80％甲醇溶液提取，采用Strata C18固相萃取小柱进行富集、净化，以甲醇-0．15％磷酸水溶液为流动相，梯度洗脱，经ZORBAX Eclipse XDB—C18色谱柱（4．6mm×150mm，5μm）分离，紫外检测器检测，检测波长为220nm，以保留时间和紫外扫描图谱定性，外标法定量。在优化实验条件下，4种植物生长调节剂的标准溶液在0．5～10mg／L范围内呈良好线性，r均大于0．999，检出限为0．05～0．15mg／kg。4种化合物在1．00、3．00mg／kg加标水平下的回收率为82％～90％，相对标准偏差均不高于3．8％。结果表明，该方法具有操作简便、回收率高、精密度好和灵敏度高等特点，适于水果中2，4-D、GA3、PP333、6-BA残留的分析。A solid phase extraction/high performance liquid chromatographic method was developed for the analysis of residues of 2, 4-dichlorophenoxyacetic acid （2, 4-D）, gibberellic acid （ GA3 ）, paclobutrazol （ PP333 ） and 6-benzylaminopurine （6-BA） residues in watermelons. Samples were extracted with 80% methanol water solution, then concentrated and cleaned up on a Strata C18 solid phase column. The separation of target compound was carried out on a ZORBAX Eclipse XDB-C18 column （4. 6 mm × 150 mm, 5 μm） under gradient condition using methano -0. 15% phosphoric acid as mobile phase. The ultraviolet detecter was used to detect analytes at 220 nm. Four plant growth regulaters were qualitatively analyzed by using retention time and UV spectra, and quantitatively determined by the external standard method. Under the optimal conditions, the calibration curves were linear between the peak areas and the concentrations in the range of 0. 5 - 10 mg/L with correlation coefficients（r） more than 0. 999. The limits of detection were between 0. 05 mg/kg and 0. 15 mg/kg. The recoveries at two spiked levels of 1.00 mg/kg and 3.00 mg/kg ranged from 82% to 90% with relative standard deviations（RSDs） no more than 3.8%. The results indicated that this method was simple, accurate and sensitive, and was suitable for the analysis of 2, 4-D, GA3, PP333 and 6-BA in fruits.

关 键 词：高效液相色谱法 固相萃取 2 4-二氯苯氧乙酸 赤霉素 多效唑 6-苄氨基嘌呤 残留 

分 类 号：O657.62[理学—分析化学] S482.8[理学—化学]