慢生型花生根瘤菌16S rRNA分析  

STUDY ON 16S rRNA OF BRADYRHIZOBIA SP. ( ARACHIS )

作　　者：陈强[1,2] 张小平[1,2] 李登煜[1,2] Terefework Z Lindstrm K 

机构地区：[1]四川农业大学农学院 [2]芬兰赫尔辛基大学

出　　处：《应用与环境生物学报》1999年第3期315-319,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金;欧盟国际合作项目

摘　　要：以２１株分离自四川、云南不同花生产区的慢生型花生根瘤菌和６株参比菌株为材料，进行了１６ＳｒＤＮＡＰＣＲＲＦＬＰ，结果除证实了慢生型花生根瘤菌同慢生型大豆根瘤菌（Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｊａｐｏｎｉｃｕｍ）高度的遗传相似性外，还发现一些菌株同Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｌｉａｏｎｉｎｇｅｎｓｉｓ和Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｅｌｋａｎｉ有较高的遗传相似性，菌株Ｓｐｒ１２的１６ＳｒＲＮＡ部分碱基序列同Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｅｌｋａｎｉ模式菌株ＵＳＤＡ７６的部分序列完全一样，从而证明我国的花生根瘤菌之间具有种水平的多样性．S rDNA PCR RFLP of 6 referent strains and 21 peanut bradyrhizobial strains isolated from different regions in Sichuan and Yunnan provinces was analyzed and the partial sequence (from 1～800 bp) of 16S rRNA of Spr1 2 was tested. The results showed that high homology in genotype among Bradyrhizobia sp.( Arachis ), B . japonicum , B . elkanii and B.liaoningensis was exited, and the partial sequence (1～800 bp) of 16S rRNA of Spr1 2 was exactly same as that of USDA76 (the type strain of B. elkanii ). Apparently, the biodiversity at the level of species is exited among Chinese peanut bradyrhizobial strains.

关 键 词：慢生型 花生根瘤菌 系统发育 16SrRNA 

分 类 号：Q939.114[生物学—微生物学]