RolB基因对三倍体毛白杨的转化研究  

Genetic Transformation of the Triploid Populus tomentosa with rolB Gene

作　　者：梁海永[1] 刘兴菊[1] 苏彦苹[1] 杨敏生[1] 

机构地区：[1]河北农业大学林学院,河北保定071000

出　　处：《西北林学院学报》2011年第2期86-90,共5页Journal of Northwest Forestry University

基　　金：国家高技术研究发展计划(863计划)(2009AA10Z107);国家林业局公益行业科研专项--杨树产业资源材培育及新品种开发关键技术研究重大项目(201004004)

摘　　要：为了提高三倍体毛白杨的生根能力,把rolB基因转入三倍体毛白杨中,获得20个转化植株,在无激素培养基上,转化植株的生根率达到100%,而未转化植株的生根率只有15%~20%。PCR扩增证明目的基因已经插入到植物的基因组中。对部分rolB基因转化植株进行了GUS组织化学染色检测,转化植株叶片中有GUS活性的表达。To improve the rooting ability of the triploid Populus tomentosa,the rolB gene was transformed into the plant and 20 transgenic clones were obtained.The in vitro rooting results showed that the rooting rate of transgenic clones recheached 100% without auxin,while only 15% to 20 % for those without transformation.PCR analysis confirmed the integration of rolB gene sequence in the transformed plants.The leaves from part of pCMB-B：GUS transformed plants were analyzed for GUS gene expression.Histochemical GUS assay demonstrated that rolB-GUS gene was mainly expressed in the leaves.

关 键 词：ROLB基因 三倍体毛白杨 转化植株 

分 类 号：S792.117[农业科学—林木遗传育种]