罗格列酮对肝癌细胞增殖的影响  

EFFECTS OF PPARγ LIGAND ROSIGLITAZONE ON PROLIFERATION OF HUMAN HEPATOCARCINOMA CELLS

作　　者：康敏[1] 钟德君[1] 杜光红[1] 任美萍[1] 

机构地区：[1]泸州医学院附属医院消化内科,四川泸州646000

出　　处：《泸州医学院学报》2011年第1期44-47,共4页Journal of Luzhou Medical College

摘　　要：目的:观察过氧化物酶体增殖物激活受体γ(PPARγ)的配体罗格列酮(RSG)对肝癌细胞增殖的影响。方法:体外培养人肝癌HepG2细胞,应用CCK-8比色法检测不同浓度RSG(25、50、100、200μmol/L)和不同作用时间RSG(24h、48h、72h)对肝癌HepG2细胞增殖的影响;流式细胞仪(FCM)检测细胞周期;实时荧光定量PCR方法分析细胞周期蛋白CyclinD1的mRNA表达变化;免疫细胞化学法分析CyclinD1的蛋白表达变化。结果:RSG抑制肝癌HepG2细胞增殖,CCK-8比色法显示增殖抑制率与RSG的浓度-时间呈正相关;FCM检测发现,RSG使肝癌HepG2细胞的细胞周期被阻滞于G1期,而进入S期的细胞明显减少,且呈浓度依赖;RSG干预后,CyclinD1的mRNA及蛋白表达在肝癌细胞中下调。结论:RSG依赖激活PPARγ途径能在体外抑制肝癌细胞生长,提示PPARγ可能是肝癌治疗的一个新分子靶点。Objective：To investigate of effect of rosiglitazone（RSG）,the ligand of peroxisome proliferator activated receptor gamma（PPARγ）,on proliferation of human hepatocarcinoma cell.Methods：Human hepatocarcinoma HepG2 cell line was cultured in vitro.The effect of RSG of different concentrations（25、50、100、200υmol·L-1）and different action times（24、48、72hours）on the growth of hepatocarcinoma cells HepG2 was detected by Cell Counting Kit-8（CCK-8） analysis.The change of cell cycle at each concentration were detected by flow cytometry（FCM）.The expression of CyclinD1 mRNA and protein were investigated by real-time fluorescent quantitive polymerase chain reaction and immunocytochemical method respectively.Results：RSG showed significant effect on inhibiting the cell proliferation.CCK-8 assay indicated that RSG could inhibit the proliferation of HepG2 cells in a concentration time dependent manner.FCM showed the cell cycle of HepG2 cells was arrested at G1 stage by RSG in a concentration dependent manner real-time fluorescent quantitive polymerase chain reaction and immunohistochemical techniques indicated the down-regulation of CyclinD1 mRNA and protein expression.Conclusion：These results indicate that the growth-inhibitory effcet of RSG is mediated through PPARγ signaling pathway in hepatocarcinoma cell in vitro,suggesting that PPARγ might be a novel therapeutic target for hepatocarcinoma.

关 键 词：罗格列酮 过氧化物酶体增殖激活受体Γ 肝癌 增殖 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]