STAT4和STAT6对日本血吸虫发育生殖及虫卵肉芽肿形成的影响  

Effects of signal transducers and activators of transcription 4 and 6 on development of worms and granuloma formation in mice infected with Schistosoma japonicum

作　　者：程喻力[1,2] 宋文剑[1] 孔争[1] 王婷[2] 刘文琪[2] 雷家慧[2] 李雍龙[2] 

机构地区：[1]江汉大学医学院病原生物学教研室,武汉430056 [2]华中科技大学同济医学院病原生物学系

出　　处：《中国血吸虫病防治杂志》2011年第1期61-64,I0002,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30771884)

摘　　要：目的观察STAT4或STAT6缺陷对日本血吸虫发育生殖及宿主虫卵肉芽肿形成的影响。方法设正常BALB/cJ小鼠及遗传背景相同的STAT4-/-、STAT6-/-小鼠3组,每组10只,每只感染日本血吸虫尾蚴25条。于感染后第42天剖杀小鼠,收集虫体,计数总虫荷及雌、雄虫荷;另取小鼠肝组织,计数肝内虫卵;制作肝组织切片,观察虫卵肉芽肿病理学变化,测量单个虫卵肉芽肿直径。结果 STAT4-/-和STAT6-/-小鼠体内总虫荷、雌雄虫配对数及肝组织中每对虫体平均产卵数与正常BALB/cJ小鼠无明显差异(P均>0.05)。STAT6-/-小鼠肝脏中单个虫卵肉芽肿直径为(213.3±68.6)μm,与感染组BALB/cJ小鼠(319.5±71.9)μm比较,差异有统计学意义(P<0.05),且虫卵肉芽肿较不典型、纤维化较不明显。STAT4-/-小鼠肝脏单个虫卵肉芽肿直径及其病理学改变与正常BALB/cJ小鼠相比无明显差异。结论 STAT4或STAT6缺陷对日本血吸虫发育生殖无明显影响。STAT6缺陷不利于虫卵肉芽肿的形成及纤维化,STAT4缺陷对虫卵肉芽肿的形成无明显影响。Objective To explore the effects of STAT4 and STAT6 on the development of worms and granuloma formation in mice infected with Schistosoma japonicum.Methods All the intact BALB/cJ mice and STAT4-/-,STAT6-/-mice with the same genetic background were infected with 25 S.japonicum cercariae.All the mice were sacrificed on the 42nd day after infection and the worms were collected.The total number of worms and the mean number of worm pairs were counted.The liver of each mouse was removed for the count of eggs,the histological examination and the determination of the size of single-egg granulomas in the liver.Results No significant differences were found in the total number of worms,the number of worm pairs and the number of eggs per pair of worms in the liver among STAT4-/-,STAT6-/-and BALB/cJ mice.The size of single-egg granulomas in the liver of STAT6-/-mice（213.3±68.6） μm was significantly smaller than that in the liver of normal BALB/cJ mice（319.5±71.9） μm（P〈0.05）.The liver granulomas were not well formed and the liver fibrosis decreased in STAT6-/-mice.Conclusions STAT4 or STAT6 deficiency has no conspicuous effect on the development and fecundity of S.japonicum.STAT6 plays an important role for the granuloma formation and liver fibrosis.

关 键 词：日本血吸虫 发育 生殖 STAT4 STAT6 

分 类 号：R383.24[医药卫生—医学寄生虫学]