“模拟钉螺”制作材料的研究  

Study on manufacturing materials of imitation snails

作　　者：陈喜珪[1] 沈定文[1] 陈鹏程[1] 覃金红[1] 石磊[2] 刘洋[2] 卢恒聪[2] 何昌浩[2] 

机构地区：[1]咸宁学院基础医学院,咸宁437100 [2]华中科技大学同济医学院病原生物学系

出　　处：《中国血吸虫病防治杂志》2011年第1期76-78,共3页Chinese Journal of Schistosomiasis Control

基　　金：湖北省教育厅自然科学基金(D200628009)

摘　　要：目的利用对日本血吸虫毛蚴有趋向作用的物质制作"模拟钉螺"吸引毛蚴。方法选用明胶、琼脂做载体材料,加入Fe3+制成"模拟钉螺"。采用改良的Roberts比较法观察"模拟钉螺"及螺狮处理过的水(SCW)对毛蚴的趋向性。结果 Fe3+浓度分别为0.1、0.5 mol/L,明胶含量分别为9%和10%的凝胶颗粒,以及Fe3+浓度为0.5 mol/L、明胶含量为9%,琼脂含量为1%或2%的混合凝胶颗粒可作为"模拟钉螺"载体,所制作的"模拟钉螺"对毛蚴的趋向性较SCW更强。结论按一定配比制成的含Fe3+的明胶以及明胶和琼脂的混合物均可作为"模拟钉螺"载体的制作材料。Objective To make imitation snails by using substances with chemotaxis to Schistosoma japonicum miracidia.Methods The imitation snails were made by using Fe3＋,gelatin and agar.The modified comparison method of Roberts was used to observe the chemotaxis of imitation snails and snail conditioned water（SCW） to Schistosoma japonicum miracidia.Results The mixture consisting of 0.1 or 0.5 mol/L Fe3＋,9% or 10% gelatin,and that consisting of 0.5 mol/L Fe3＋,9% gelatin,2% or 1% agar could be used to make the imitation snails.The chemotaxis of imitation snails made by the mixtures above was stronger than that of SCW.Conclusion The mixtures made by certain proportion of Fe3＋,gelatin or gelatin and agar can be used to make imitation snails.

关 键 词：日本血吸虫 毛蚴 “模拟钉螺” 载体材料 

分 类 号：R383.24[医药卫生—医学寄生虫学]