核黄素结合紫外光灭活血小板细菌及膜糖蛋白的变化  

Effect on bacteria inactivation using riboflavin as a photosensitizer and detect membrane glycoproteins after treatment

作　　者：卫玉芝[1] 许伟[1] 钟涛[1] 张循善[1] 

机构地区：[1]安徽医科大学第一附属医院输血科,安徽合肥230022

出　　处：《安徽医药》2011年第1期80-81,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨核黄素光化学法灭活单采血小板污染菌的效果及其膜糖蛋白表达的变化情况。方法将14ml浓度为500μmol.L-1核黄素加到125ml的单采血小板悬液中,再将一定浓度的G+菌(表皮葡萄球菌ATCC12228)和G-菌(大肠埃希氏菌ATCC25922)分别注入上述混悬液中,经(265～370nm)广谱紫外光(6.2J.ml-1)照射8～10min后,测定其浓度,观察灭活效果,并用流式细胞仪检测血小板膜糖蛋白的变化情况。结果经浓度50μmol.L-1的核黄素结合强度为6.2J.ml-1紫外光照射8～10min,可将一定浓度的模型菌灭活至<1.22 logCFU/ml。结论核黄素结合紫外光照射可以有效灭活细菌,而单采血小板的CD62p、PAC-1的阳性表达率和阴性对照比较差异均无显著性。Aim To explore a photochemical method using riboflavin plus UV light that can inactivate selected model bacteria and to detect its membrane glycoproteins.Methods 500 μmol·L-1 riboflavin（14 ml）was added to the apheresis platelet（125 ml）blood bag,then model bacteria（Staphylococcus epidermidis ATCC12228 and Escherichia coli ATCC25922）were added to apheresis platelet,and broadband UV light was used illuminate the blood bag,before the bacteria titer was measured.The inactivation effects of UV light plus riboflavin for bacteria was studied.The impact of a new technology for pathogen reduction based on riboflavin plus illumination at 6.2 J·ml-1 on functional and biochemical characteristics of PLT was evaluated.Its GP CD62p and PAC-1 were detected by flow cytometry.Results With a riboflavin concentration of 50 μmol·L-1 and UV light 265~370 nm intensity of 6.2 J·ml-1,mode bacteria in apheresis platelet blood bag could be reduced to less than 1.22 logCFU/ml in 10 minutes.Conclusions Riboflavin plus ultraviolet light could significantly inactivate selected bacteria.Its membrane glycoproteins CD62p and PAC-1 was adequately maintained after treatment and during storage,this was not beyond accepted levels.

关 键 词：核黄素/药理学 光敏感药药理学 细菌灭活 膜糖蛋白 

分 类 号：R457.1[医药卫生—治疗学]