TNFRSF1B基因多态性与冠心病的相关分析  

Single nucleotide polymorphism in tumor necrosis factor receptor super family member 1B(TNFRSF1B) and coronary heart disease:A correlation study

作　　者：任俊峰[1] 李俊男[1] 苏锐[1] 边云飞[1] 

机构地区：[1]山西医科大学第二医院心内科,太原030001

出　　处：《中国药物与临床》2011年第2期128-131,共4页Chinese Remedies & Clinics

基　　金：国家自然基金项目(20090311057-4)

摘　　要：目的研究中国人群TNFRSF1B基因多态性与冠心病的关系。方法选取冠心病患者416例和匹配对照组208例,记录所有研究对象的病史、体格检查等临床资料及其他流行病学资料,采用聚合酶链反应和连接酶检测反应(PCR-LDR)的方法检测各组TNFRSF1B基因6号外显子rs1061622SNPs位点等位基因A/G的基因型并统计各组的多态性频率。结果 rs1061622的TT和GG等位基因和基因型频率冠心病组显著高于对照组(冠心病组:TT为59.2%,TG为34.8%,GG为6.0%;对照组:TT为32.6%,TG为64.4%,GG为2.8%;P=0.008)。并在校正了年龄、性别、高血压病史、糖尿病史、甘油三酶和胆固醇等传统的危险因素影响后,这种相关性依然存在。结论 TNFRSF1B基因rs1061622多态性TT和基因型是中国汉族人群冠心病的危险因素。Objective To investigate the correlation between the single nucleotide polymorphism（SNP） of TNFRSF1B gene and coronary heart disease（CHD） in Chinese population.Methods Four hundred and sixteen CHD patients（CHD group） and 208 matched controls（control group） were selected from the Second Hospital of Shanxi Medical University and recorded for their clinical data including medical history,physical workup and other epidemiological data.The polymerase chain reaction-ligase detection reaction（PCR-LDR） was used to detect the allelic gene（A/G） genotype in the exon 6 of TNFRSF1B（rs1061622SNPs） of both the two groups.The frequency of polymorphism was then calculated.Results The genotype frequency of both TT and GG alleles（rs1061622） was significantly higher in CHD group than that in the control groups（CHD group：TT 59.2%,TG 34.8%,GG 6.0%;Control group：TT 32.6%,TG 64.4%,GG 2.8%,P=0.008）.This correlation existed even after adjustment for conventional risk factors such as age,gender,hypertension,diabetes,triglyceride and cholesterol.Conclusion The SNP and genotype of TT in TNFRSF1B gene（rs1061622） are risk factors of CHD in Chinese Han population.

关 键 词：肿瘤坏死因子 多态性 基因 冠状动脉硬化 

分 类 号：R541.4[医药卫生—心血管疾病]