化痰与活血方对非酒精性脂肪肝氧应激的影响  

Experimental study on effect of Huatan,Huoxue decoctions on oxidative stress in non-alcoholic fatty liver rats

作　　者：夏蓉[1] 车念聪[1] 袁梦[1] 季巍巍[1] 法振鹏[1] 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《中华中医药杂志》2011年第2期260-263,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市教育委员会科技计划面上项目(No.KM200810025028)~~

摘　　要：目的:观察化痰方与活血方对非酒精性脂肪肝(NAFLD)大鼠氧应激损伤的影响,探讨两方在NAFLD治疗中的作用。方法:制备NAFLD大鼠模型,同时用化痰方和活血方对模型进行干预,并与西药组(二甲双胍+罗格列酮)进行对照,设置正常组、模型组、西药组、化痰方组、活血方组及合方组共6组,检测肝组织CYP450及CYP2E1活性,测定SOD、MDA。结果:模型组的CYP450和CYP2E1活性增强(P<0.05),SOD活性下降(P<0.05),MDA含量升高(P<0.01);所有中药组均能降低CYP2E1活性(P<0.05),而西药组能降低CYP450活性(P<0.05);化痰方与活血方能够提高SOD活性(P<0.01),降低MDA含量。结论:NAFLD大鼠存在明显的氧应激和脂质过氧化损伤情况,化痰方与活血方均能不同程度地调节NAFLD大鼠的氧应激水平,改善脂质过氧化,对NAFLD具有确切的防治作用。Objective：TO observe the effect of Huatan decoction and Huoxue decoction on oxidative stress in rat model of non-alcoholic fatty liver disease,discussing the efficacy of these two decoctions in the prevention and treatment of NAFLD.Materials and Methods： Experimental animal model of NAFLD were established,treated with Huatan,Huoxue decoctions,and compared with the control group,the model group and the treatment group in which rats were treated with insulin sensitizer-Metformin and Rosiglitazone.Test CYP450,CYP2E1 activity in hepatic tissue,and observe the change of SOD,MDA.Results： CYP450,CYP2E1 activity（P0.05） increased in model group,and SOD（P0.05） decreased,while the content of MDA（P0.05） increased.All Chinese groups（CMG） could decrease CYP2E1 activity（P0.05）,while the western group（WMG） can reduce CYP450 activity（P0.05）.Compared with model group,in Huatan and Huoxue groups,the SOD activity decreased obviously（P0.01）,besides WMG and Huatan decoctions could reduce the content of MDA（P0.05）,Huoxue group had a lower,but group difference was not significant.Conclusion： NAFLD rats had obvious oxidative stress and lipid peroxidation damage.Huatan,Huoxue decoctions can modulate the level of oxidative stress,and improve lipid peroxidation.They had precise control on the role of NAFLD.

关 键 词：非酒精性脂肪肝 氧应激 化痰方 活血方 实验研究 

分 类 号：R285.5[医药卫生—中药学]