野生核桃楸树皮中黄酮类成分的高效液相色谱-质谱法分析  

High performance liquid chromatography-mass spectrometry analysis of flavonoids in Juglans mandshurica Maxim.stem-barks

作　　者：张咏莉[1] 林桂芳 崔玉强[1] 詹苗[1] 冀军锋 

机构地区：[1]广东药学院基础学院生物教研室,广州510006 [2]中山市火炬开发区医院药剂科,中山528437 [3]中山市尤利卡天然药物有限公司,中山528437

出　　处：《中华中医药杂志》2011年第2期369-371,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中山市健康产业基地专项项目(No.2009H021);广东省社会发展科技计划项目(No.83046)~~

摘　　要：目的:制备和分析野生核桃楸树皮水提物中的黄酮类成分。方法:采用碱溶酸沉法提取核桃楸树皮水提物中的黄酮类化合物,硅胶柱层析法进行分离纯化,采用液相色谱-质谱法(liquid chromatography-mass spectrometry,LC-MS)检测黄酮类化合物的成分。结果:由色谱图可以看出,保留时间为4.235s和4.955s时物质的相对含量为62.00%和10.63%,为该黄酮类提取物的2个主要成分。由质谱图可以推断,其含有的主要成分为甲氧基黄酮和柚皮素。结论:液相色谱-质谱法对于快速分析野生核桃楸树皮水提物中的黄酮类活性成分具有可行性。Objective： To extract and analyze flavonoids in aqueous extract of Juglans mandshurica Maxim.stem-barks.Methods： The processes of alkaline dissolution-acid sediment and silica gel column chromatography were applied to extract flavonoids in Juglans mandshurica Maxim.stem-barks,and the structure of flavonoids were identified by high performance liquid chromatography-Mass Spectrometry（LC-MS）.Results： The results of chromatography analysis indicated that the two bases relative concentration of relative retention time 4.23 and 4.95 were 62.00% and 10.63% respectively,and we can presume that the bases were methoxyflavone and naringenin by mass spectrum.Conclusion： This method can be available and helpful to find or analysis the flavonoids constituents from the aqueous extract of Juglans mandshurica Maxim.stem-barks.

关 键 词：核桃楸树皮 水提物 黄酮 液相色谱-质谱法 

分 类 号：R284[医药卫生—中药学]