前体物对红豆杉培养细胞中紫杉醇生物合成的影响(英文)  

EFFECTS OF PRECURSORS ON TAXOL BIOSYNTHESIS IN CELL CULTURES OF TAXUS CHINENSIS

作　　者：李家儒[1] 曹孟德[1] 刘曼西[2] 陈辉蓉[1] 吴振斌[1] 王君健[2] 

机构地区：[1]中国科学院水生生物研究所,武汉430072 [2]华中理工大学生物工程系,武汉430074

出　　处：《植物研究》1999年第3期356-360,共5页Bulletin of Botanical Research

摘　　要：本文报道了添加７ 种紫杉醇前体物／调节物后，红豆杉（ Ｔ．ｃｈｉｎｅｎｓｉｓ（Ｐｉｌｇｅｒ） Ｒｅｈｄ）ＴＣ１５８ 细胞系的反应。在红豆杉细胞悬浮培养２５ 天时，分别加入不同浓度乙酸钠、苯甲酸钠、Ｌ－ 苯丙氨酸、甘氨酸、丝氨酸、α－ 蒎烯、松节油。试验结果表明，各前体物对红豆杉细胞生长无明显影响。The response of T. chinensis TC158 cell line to the addition of precursors/ regulators of taxol is described in this paper. On the 25th day after inoculation, cell cultures were supplemented with different concentrations of one of the following compounds: sodium acetate, sodium benzoate, L-phenylalanine, glycine, L-serine, α- pinene and turpentine oil. The results demonstrated that taxol biosynthesis was improved in different degrees, while the cell growth was not significantly affected by various precursors / regulators.

关 键 词：前体物 紫杉醇 生物合成 细胞培养 红豆杉 

分 类 号：Q944.6[生物学—植物学] TQ464.2[化学工程—制药化工]