小鼠扩张囊胚颗粒法玻璃化冷冻保存技术研究  

Cryopreservation of Expanded Mouse Blastocysts in Pellets by Vitrification

作　　者：朱士恩[1] 曾申明[1] 张忠诚[1] 

机构地区：[1]中国农业大学动物科技学院

出　　处：《上海实验动物科学》1999年第2期67-71,共5页Shanghai Laboratory Animal Science

摘　　要：在２５℃室温下，用ＥＦＳ３０玻璃化溶液，对小鼠扩张囊胚进行颗粒法玻璃化冷冻保存。结果，胚胎在ＥＦＳ３０溶液中平衡１ｍｉｎ后滴入液氮中，解冻后的胚胎发育率达１００％，囊胚孵化率达７６％。而在１０％乙二醇溶液中将胚胎预处理５ｍｉｎ，再移入ＥＦＳ３０中平衡１ｍｉｎ后冷冻保存的胚胎，解冻后的发育率和囊胚孵化率分别为９８％和６３％。上述２组的胚胎发育率与对照组无明显差异，而囊胚发育率均明显低于对照组（Ｐ＜０．０１）。上述２组胚胎解冻后植入假孕小鼠子宫后的受体妊娠率分别为９０％和８１％，产出率分别为７１％和５８％，与对照组（６１％）相比均无显著性差异（Ｐ＞０．０５）。The cryopreservation of expanded mouse blastocysts in pellets by vitrific ation was studied at 25℃ with EFS30 solution (contains 21% ficoll,0.35mol/L suc rose and 30% ethylene glycol) as a cryoprotectant.The expanded blastocysts in gr oup 1 were equilibrated in EFS30 solution for 1 min,then pluged into liquid nitr ogen,while in group 2 they were pretreated with 10% ethylene glycol for 5 min and equilibrated in EFS30 solution for 1 min,then pluged into liquid nitrogen.The d evelopment rate and the hatch rate of blastocysts in group 1 were 100% and 70% r espectively after being thawed and cultured,while in group 2 they were 98% and 6 3% respectively.The development rates in group 1 and 2 didn't differ significan tly from that of control (P>0.05).The percentages of pregnant dams a nd fullterm pregnancy offsprings were 90% and 71% respectively in group 1, while in group 2 they were 81% and 58% respectively,after the thawed expanded bl astocysts were transplanted into pseudopregnant mice.There were no significant differences in the percentages of pregnant dams and fullterm pregnancy offsprings between the cryopreserved groups and the control (P> 0.05).

关 键 词：冷冻保存 玻璃化 颗粒法 扩张囊胚 保存 小鼠 

分 类 号：S814.6[农业科学—畜牧学]