碱性盐胁迫对碱地风毛菊苗期脯氨酸代谢途径的影响  

Effect of Alkaline Salts on Proline Metabolism of Saussurea runcinata at Seedling Stage

作　　者：夏方山[1] 董秋丽[1] 董宽虎[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国草地学报》2011年第1期48-53,共6页Chinese Journal of Grassland

基　　金：国家"十一五"科技支撑计划项目(2007BAD56B01)资助

摘　　要：以Na2CO3和NaHCO3对苗期碱地风毛菊(Saussurea runcinata)进行胁迫,探讨其苗期耐碱性及脯氨酸代谢途径。结果表明:NaHCO3和Na2CO3胁迫下,碱地风毛菊中脯氨酸含量增加,最大值为对照的15.5倍;吡咯啉-5-羧酸合成酶(P5CS)活性受到抑制;鸟氨酸δ-氨基-转移酶(δ-OAT)活性升高,最大为12.58 U/μg Protein;碱地风毛菊中脯氨酸合成途径以鸟氨酸(Orn)途径为主;NaHCO3胁迫下脯氨酸降解代谢旺盛,Na2CO3胁迫下,碱地风毛菊叶片中脯氨酸脱氢酶(ProDH)活性下降,根系中ProDH活性增大,脯氨酸降解代谢旺盛。The experiment was carried out to assess the proline metabolism and salinity tolerance of Saussurea runcinata seedling under varying levels of Na2CO3 and NaHCO3（0,30,60,90,120,150 and 180 mmol/L）.The results showed that the proline content in Saussurea runcinata seedlings increased significantly under Na2CO3 and NaHCO3 stress,P5CS activity was inhibited,but δ-OAT activity was enhanced,the proline metabolism synthesis pathway in Saussurea runcinata seedlings was mainly the pathway of ornithine;the proline degradation of was active under NaHCO3 stress,ProDH activity in leaves of Saussurea runcinata was declined under Na2CO3 stress,ProDH activity in roots was increased,and proline degradation was active.

关 键 词：脯氨酸代谢 脯氨酸 δ-氨基转移酶 吡咯啉-5-羧酸合成酶 脯氨酸脱氢酶 

分 类 号：Q945.78[生物学—植物学]