KM小鼠与BALB/c小鼠五项免疫学指标的初步观察  

Observetion on Several Immunologic Parameters\= in Kunming Mice and BALB/c Mice

作　　者：卢笑丛[1] 孙凡中[1] 田辉[1] 戴涌 唐利军[1] 林乔[2] 王玉娥[2] 冯玲玲[2] 

机构地区：[1]湖北省医学实验动物中心 [2]湖北省医学科学院病毒所

出　　处：《实验动物科学与管理》1999年第2期11-13,共3页Laboratory Animal Science & Administration

摘　　要：采用五种免疫功能测定法对８３２只健康ＫＭ小鼠和８３只ＢＡＬＢ／ｃ小鼠进行免疫学功能指标的测定。结果表明ＣｏｎＡ诱导小鼠脾淋巴细胞转化能力ＯＤ值分别为００１４８～００１９２和００１２０１～００２０６；二硝基氟苯诱导小鼠迟发型变态反应（耳肿胀法）小鼠耳重差分别为１０３９～１２１７ｍｇ和１１４６８～１６２３４ｍｇ；血清溶血素抗体积数分别为７８９８～９１３０和１０６７３～１１６０７；碳廓清吞噬指数ａ分别为４７５２～４８５８和４４７０～５５９８；小鼠巨噬细胞吞噬鸡红细胞的指数分别为０３９８～０４６１和０３３１～０４９９，吞噬百分数分别为３３７２～３７１８和２９２４～４１１０。上述数据可信区间为９９％，两鼠间ＤＴＨ及抗体积数值有高度显著性差异（Ｐ＜００１）。在进行免疫药理学、免疫毒理学和保健食品功能学研究时，以实验小鼠为材料运用ＤＴＨ和血清溶血素抗体积数实验时，应首选ＢＡＬＢ／ｃ小鼠。而进行其它三项免疫学指标实验时可首选ＫＭ小鼠。Determination paramters of immunologic functiions by five methods in 832 Kunming mice and 83 BALB/c mice was conducted. The results showed that the OD is 0.01480.0192 in KM and 0.012010.0206 in BALB/c in transfer ability of splenocytes by ConA irradiated mice; ear\|weight\|deviation of mice is 10.3912.17mg in Km, 11.46816.234 in BALB/c in delayed type hypersensitivity (DTH) by dinitrofluorobenzene (DNFB) irradiated mice; antibody integral of sero hemolysin is 78.9891.30 in KM and 106.73116.07 in BALB/c; clearance index of charcoal particles (a) in KM and BALB/c are 4.7514.858 and 4.4705.598 respectively; phagocytosis index of murine peritoneal macrophage is 0.3980.461 in KM, 0.3310.499 in BALB/c and phagocytosis percentage (%) is 33.7237.18 in KM and 29.2441.10 in BALB/c. The confidence limited of all of above parameters are 99%. DTH and antibody integral of sero hemolysin in KM and BALB/c are significant deference (P<0.01). It suggested that normal index of immunolgic function could provid basic valuble data for study of immunology, pharmacology and health foods, also perfect biological intension of Kunming mice. During the study of DTH and antibody integral of sero\|hemolysin in immunopharmacolgy, immunotoxinology and health foods, BALB/c mice should be first selected as experimental material. Otherwise, to detect other three immunologic indexs should be first selected KM mice.

关 键 词：小鼠 KM小鼠 BALB/C小鼠 免疫测定 

分 类 号：S852.4[农业科学—基础兽医学] S858.9[农业科学—兽医学]