白芍总苷对腹主动脉结扎所致大鼠心肌重构的影响  

Experimental Researches of Glucosides of Radix Paeoniae Alba on Reversing Myocardial Remodeling Induced by Abdominal Aortic Ligation(Partial Occlusion)

作　　者：韩蕾[1,2] 周晓辉[3] 王维伟[4] 陈长勋[5] 

机构地区：[1]上海中医药大学,2007级博士研究生上海201203 [2]江苏建康职业学院,江苏南京210029 [3]中国药科大学临床药学教研室,江苏南京211198 [4]辽宁中医药大学 [5]上海中医药大学中药学院药理教研室,上海201203

出　　处：《中华中医药学刊》2011年第2期330-334,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省教育厅科学技术研究项目资助(2008438);中国药科大学引进人才启动基金项目(01211100)

摘　　要：目的:用白芍总苷作用于腹主动脉结扎所致的大鼠心肌重构整体动物模型,观察白芍中苷类成分的抗心肌重构作用,分析其可能的作用机理,以期为其抗心肌重构的可能临床应用提供实验依据。方法:不完全结扎大鼠腹主动脉造成压力超负荷型心肌重构模型,假手术组动物除不进行结扎外其他操作与模型动物相同。造模一周后取存活的造模动物随机分为模型对照组、阳性对照药卡托普利45mg/(kg.d)组、白芍总苷100mg/(kg.d)组、白芍总苷200mg/(kg.d)组。各组动物灌胃给予受试药物或等体积蒸馏水20mL/(kg.d),每天给药1次,连续给药30天。末次给药动物禁食不禁水12h,进行如下指标的检测:颈总动脉插管测定血流动力学相关指标;称取体重、摘取心脏测定左心室指数及全心指数。结果:腹主动脉不完全结扎1个月,模型组动物的左心室指数、全心指数明显升高(P<0.01),收缩压、平均压明显升高(P<0.05)。与模型对照组相比,阳性药卡托普利45mg/(kg.d)可使左心室指数、全心指数明显降低(P<0.01),收缩压明显降低(P<0.05);白芍总苷100mg/(kg.d)可使左心室指数明显降低(P<0.05);白芍总苷200mg/(kg.d)可使左心室指数、全心指数明显降低,平均压降低(P<0.05)。结论:白芍总苷灌胃给药具有一定的对抗腹主动脉结扎所致心肌重构作用,其中的芍药苷可能是白芍总苷抗心肌重构的主要药效学成分。Objective ： This study was designed to investigate the attenuate effect of TGP on myocardial remodeling in- duced by abdominal aortic ligation （partial occlusion） in vivo. Methods： Pressure overload myocardial remodeling rat model was established by partial occlusion of abdominal aorta. Sham - operated rats underwent the same procedure except ligations were used as sham - operated control. One week after operation, the surviving model rats were randomly divided into 4 groups： model control group, captopril treated group 45mg/（kg·d）, TGP treated group 100mg/（kg·d）, and TGP treated group 200mg/（kg·d）. Drugs were taken orally for 50 days. At the end of the experiment, hearts were taken, left ventricular weight index （LVW/BW） and heart weight index （HW/BW） were measured. Hemedynamic parameters were measured by carotid artery cannulation. Results： One month after the ligation, LVW/BW, HW/BW and serum IL - 1β content of the model group were increased remarkably （ compared with that of the sham - operated group, P 〈0.01）. SBP and MAP were increased （ P 〈0.05）. Compared with the control solution, captopril 45mg/kg/d administration decreased LVW/BW,HW/BW remarkably （ P 〈 0.01 ）. SBP were also decreased （ P 〈 0.05 ）. TGP 100mg/kg/d administration decreased LVW/HW （ P 〈 0.05 ）. TGP 200mg/（kg·d） administration decreased LVW/ HW, HW/BW, MAP remarkably （ P 〈 0.05 ）. Conclusions ： According to the results mentioned above, we can conclude that TGP can attenuate myocardial remodeling induced by abdominal aortic ligation （partial occlusion）. PAE may be the main effective component of TGP.

关 键 词：白芍总苷 芍药苷 心肌重构 

分 类 号：R285.5[医药卫生—中药学]