阿托伐他汀对2型糖尿病患者外周血内皮祖细胞功能的影响及其机制的初步探讨  

Effect and mechanism of atorvastatin on endothelial progenitor cells from peripheral blood in patients with type 2 diabetes

作　　者：马兰兰[1] 石丽娟[1] 

机构地区：[1]中国医科大学基础医学院,110001

出　　处：《山西医药杂志（上半月）》2011年第2期124-126,共3页Shanxi Medical Journal

基　　金：辽宁省高等学校科研基金(2008840)

摘　　要：目的观察阿托伐他汀对2型糖尿病患者(T2DM)患者外周血内皮祖细胞功能的影响并初步探讨其机制。方法将40例糖尿病患者随机分为他汀治疗组和对照组,前者给予阿托伐他汀40 mg/d口服,治疗前及治疗后2、4周抽取外周血,采用密度梯度离心法分离培养内皮祖细胞,应用噻唑蓝(MTT)法检测内皮祖细胞增殖情况,Transwell小室检测迁移,Matrigel管腔形成实验检测管腔形成能力。另取20例T2DM患者血培养内皮祖细胞,加入阿托伐他汀、一氧化氮合酶(NOS)/NO途径抑制剂L-NAME后观察对其功能的影响。结果阿托伐他汀治疗后2周、4周外周血内皮祖细胞数量明显上升,功能明显改善(P<0.01)。体外实验他汀+L-NAME组的血内皮祖细胞数目较他汀组明显下降(P<0.01)。结论阿托伐他汀对糖尿病患者内皮祖细胞的功能具有一定保护作用,这种作用能被L-NAME阻断。阿托伐他汀的作用机制可能与激活NOS/NO信号通路有关。Objective To investigate the effect of atorvastain on the function of endothelial progenitor cells （EPCs） group and control group. Methods The former were treated with 40 mg of atorvastain per day for 4 weeks. Before , 2 weeks and 4 weeks after the statin therapy, mononuclear cells were isolated from blood of patients by density gradient centrifugation. Adherence cells were harvested after 7 days culture. EPCs were characterized by laser scanning confocal microscope. EPCs proliferation, transfer and blood vessels formation were detected with MTT eolorimetry, Transwell chamber and Matrigel. EPC harvested from other 20 patients blood were incubated with or without atorvastain and L-NAME, a specific inhibitor of NOS. Then the function of EPCs was investigated. Results The number and function of EPCs remarkably improved in subjects with atorvastain in 2 and 4 weeks （ P〈0.01）. The number of EPCs decreased （ P〈0.05）after to add L-NAME in vitro experiment in atorvastain group. Conclusion Atorvastain can improve the function of EPCs and this effect may be inhibited by L- NAME.

关 键 词：糖尿病 2型 内皮祖细胞 阿托伐他汀 

分 类 号：R587.1[医药卫生—内分泌]