止血带休克时主动脉舒缩功能与一氧化氮合酶活性的关系  

THE RELATIONSHIP BETWEEN THE AORTIC NITRIC OXIDE SYNTHASE ACTIVITY AND VASCULAR FUNCTION IN TOURNIQUET SHOCK

作　　者：张连元[1,2] 张之玮[1,2] 董淑云 郑素琴[1,2] 苏静怡[1,2] 唐朝枢 

机构地区：[1]华北煤炭医学院病理生理学教研室 [2]北京医科大学心血管基础研究所

出　　处：《中国应用生理学杂志》1999年第2期104-106,共3页Chinese Journal of Applied Physiology

摘　　要：目的：为探讨止血带休克发生过程中血管舒缩功能的改变及意义，以及与血管一氧化氮（ＮＯ）产生的关系。方法：采用Ｒｏｓｅｎｔｈａｌ方法复制大鼠止血带休克（ＴｏＳ）模型，分别测定对照组和ＴｏＳ组大鼠血浆中，主动脉孵育液中亚硝酸盐（ＮＯ－２）含量及主动脉组织中ｃＧＭＰ含量，一氧化氮合酶（ＮＯＳ）活性，同时观察了主动脉血管环舒缩功能的改变。结果：ＴｏＳ大鼠离体主动脉环对去甲肾上腺素的反应性降低；其血浆和主动脉孵育液中ＮＯ－２明显增加；主动脉ｃＧＭＰ含量增多；主动脉血管总ＮＯＳ活性加强，其中主要是诱导型ＮＯＳ（ｉＮＯＳ）活性增加。结论：ＴｏＳ大鼠主动脉ＮＯＳ活性加强，产生ＮＯ增多，造成血管低反应性。Abstract Aim:To investigate the significance and the change of vascular function and the relation between nitric oxide(NO) production and function of the vascular in the tourniquet shock(ToS).Methods:This work was done on rat ToS model by Rosenthal way. The NO - 2content in the plasma and aorta incubation and cGMP content and nitric oxide synthase(NOS) activity in the aortic tissue was determined. At the same time, the function of aortic ring was observed. Results: The reactivity of isolated perfused aortic ring to noradrenaline decreased; the NO - 2 content in the plasma and aorta incubation increased; the cGMP content in the aortic tissue increased too; the total NOS activity, especialy inducible NOS(iNOS) activity, increased in the ToS rats. Conclusion:In the ToS rats the vascular hyporeactivity is caused by increasing nitric oxide production which is endothelium undepend tourniquet shock; aorta; nitric oxide; nitric oxide synthase

关 键 词：止血带休克 一氧化氮合酶 主动脉舒缩功能 

分 类 号：R605.971[医药卫生—急诊医学] R654.402[医药卫生—外科学]