大鼠海马CA_1区锥体细胞的急性分离和鉴定  

ACUTE ISOLATION AND IDETIFICATION OF CA 1 PYRAMIDAL NEURONS IN HIPPOCAMPUS OF RATS

作　　者：许长庆[1,2,3,4] 张宗明 夏作理[1,2,3,4] 吴才宏[1,2,3,4] 周培爱 

机构地区：[1]广东省江门市中心医院神经内科 [2]北京医科大学附属三院外科 [3]泰山医学院附属医院老年病科 [4]北京大学生物膜与膜生物工程国家实验室

出　　处：《中国应用生理学杂志》1999年第2期189-192,共4页Chinese Journal of Applied Physiology

基　　金：山东省自然科学基金

摘　　要：目的：建立一种适用膜片钳技术的大鼠海马ＣＡ１区锥体细胞的急性分离方法，并对其进行鉴定。方法：采用酶加机械分离法制备７～１０ｄ鼠龄的大鼠海马ＣＡ１区锥体细胞，用免疫荧光技术鉴定神经元，其电生理学特性测定用全细胞膜片钳技术。结果：分离的锥体细胞是海马ＣＡ１区锥体细胞，且具有正常的电生理学特性，并保存了某些主要的电压依赖性和受体依赖性离子通道特性。结论：成功地建立了一种简单、快速、高产的适用于膜片钳技术的大鼠海马ＣＡ１区锥体细胞的急性分离方法。Aim: To establish a method for acute isolation of rat hippocampal CA 1 pyramidal neurons (RHCPN) suitable for patch clamp techniqne (PCT), and to identify the characteristics of RHCPN. Methods: RHCPN were obtained by a combination of mechanical and enzymatic means from 7 to 10 day old rats. PCT were used to measure the electrophysiological properties of RHCPN and neurons were identified by inmmunofluorescien method. Results: Acutely isolated RHCPN were easy to be recorded by wholl cell PCT. They had well preserved electrical and chemical membrane properties of some major voltage dependent and receptor gated ionic channels. Conclusions: A simple, fast and high yielded method for acutely isolating RHCPN suitable for PCT was successfully found.

关 键 词：海马 锥体细胞 急性分离 方法 膜片钳 电生理学 

分 类 号：R338.8[医药卫生—人体生理学] R338.26[医药卫生—基础医学]