中枢应用β-内啡肽对大鼠血浆唾液酸水平的影响以及与免疫功能的关系  

EFFECT OF β ENDORPHIN ON PLASMA SIALIC ACID LEVEL IN RATS AND THE RELATIONSHIP WITH THE IMMUNE FUNCTION 

作　　者：陈文芳[1,2] 陈蕾[1,2] 吕秀文[1,2] 陈家津[1,2] 

机构地区：[1]青岛大学医学院生理教研室 [2]青岛大学医学院附属医院检验科

出　　处：《生理学报》1999年第3期253-257,共5页Acta Physiologica Sinica

基　　金：山东省教委科研基金

摘　　要：实验选择体重２００～２５０ｇ健康Ｗｉｓｔａｒ大鼠６４只，采用麻醉大鼠中枢微量注射、分光光度测定法及免疫组织化学法，研究侧脑室、弓状核（ＡＲＣ）区注射β内啡肽（βＥＰ）对大鼠血浆唾液酸（ＳＡ）水平的影响及与免疫功能的关系。结果表明：（１）侧脑室注射βＥＰ可明显降低血浆ＳＡ水平；（２）血浆ＳＡ水平在ＡＲＣ区注射βＥＰ后明显降低，此效应可被Ｍ胆碱受体阻断剂阿托品或切断双侧颈迷走神经所阻断；（３）ＡＲＣ区注射βＥＰ后，外周血ＣＤ３，ＣＤ４明显升高，ＣＤ８明显降低，ＣＤ４／ＣＤ８比值升高。以上结果提示，中枢应用βＥＰ可降低血浆ＳＡ水平，ＡＲＣ可能是主要的中枢作用部位，其机制与Ｍ胆碱能受体密切相关，可能是通过副交感神经实现此效应。Abstract With the use of β EP microinjection into the central nervous system, spectrophotometrical analysis and immunochemical method we studied the effect of β Endorphin (β EP) on plasma sialic acid (SA) level. The results indicate: (1) The plasma SA level was significantly decreased after microinjection of β EP into the lateral ventricle (LV); (2) When β EP was microinjection into nucleus arcuatus (ARC), the plasma SA level was significantly decreased ( P <0 05). This effect can be blocked by i v atropine or vagotomy; (3) Microinjection of β EP into ARC can cause the increase of CD3, CD4 and CD4/CD8, while CD8 was decreased. The above results suggest that the possible mechanism underlying the decrease of plasma SA level is mediated by cholinergic muscarinic receptor, and the cellular immune function is enhanced.

关 键 词：神经中枢 血浆 唾液酸 Β-内啡肽 免疫功能 

分 类 号：Q426[生物学—神经生物学] Q46[生物学—生理学]