水稻生活精细胞的分离及细胞学观察  被引量:8

Isolation and Cytological Observation of Viable Sperm Cells of Rice 

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作  者:苟小平[1] 王胜华[1] 陈放[1] 

机构地区:[1]四川大学生命科学学院

出  处:《Acta Botanica Sinica》1999年第6期669-671,共3页Acta Botanica Sinica(植物学报:英文版)

基  金:欧洲委员会国际合作项目

摘  要:近10多年来已经成功分离得到白花丹[1]、玉米[2,3]、百合[4]等植物的精细胞,并建立起玉米的离体受精实验体系[5,6]。但水稻作为我国和世界重要的粮食作物,迄今未见有类似工作的报道。本文采用渗透压冲击法成功分离得到水稻的生活精细胞。1材料和方法...Sperm cells of rice ( Oryza sativa L.) were isolated from mature pollen grains near anthesis using osmotic shock with sucrose solution. They were separated from pollen contaminants by a Percoll gradient centrifugation. Isolated sperm cells were viable as tested with the fluorochromatic reaction. Cytological observations revealed that the two sperm cells just released from a pollen grain were linked together and each had a long tail_like extension. The isolated sperm cells were spherical with irregular surface and most of them separated, but some still remained in pairs that connected by finger_like structure. As observed with TEM, the isolated sperm cell was surrounded by a single plasma membrane and cell wall did not exist. Sperm nucleus was large and euchromatic.

关 键 词:水稻 精细胞 分离 形态结构 

分 类 号:S511.032[农业科学—作物学]

 

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