检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]四川大学生命科学学院
出 处:《Acta Botanica Sinica》1999年第6期669-671,共3页Acta Botanica Sinica(植物学报:英文版)
基 金:欧洲委员会国际合作项目
摘 要:近10多年来已经成功分离得到白花丹[1]、玉米[2,3]、百合[4]等植物的精细胞,并建立起玉米的离体受精实验体系[5,6]。但水稻作为我国和世界重要的粮食作物,迄今未见有类似工作的报道。本文采用渗透压冲击法成功分离得到水稻的生活精细胞。1材料和方法...Sperm cells of rice ( Oryza sativa L.) were isolated from mature pollen grains near anthesis using osmotic shock with sucrose solution. They were separated from pollen contaminants by a Percoll gradient centrifugation. Isolated sperm cells were viable as tested with the fluorochromatic reaction. Cytological observations revealed that the two sperm cells just released from a pollen grain were linked together and each had a long tail_like extension. The isolated sperm cells were spherical with irregular surface and most of them separated, but some still remained in pairs that connected by finger_like structure. As observed with TEM, the isolated sperm cell was surrounded by a single plasma membrane and cell wall did not exist. Sperm nucleus was large and euchromatic.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.28