芸豆植物凝集素对小鼠体外受精的影响  

Effect of phytohemagglutinin on in vitro fertilization in mice

作　　者：张丽芬[1] 王昌梅[1] 杨明洁[1] 张田[1] 王敏康[1] 

机构地区：[1]云南师范大学生命科学学院,昆明650092

出　　处：《中国计划生育学杂志》2011年第2期81-85,共5页Chinese Journal of Family Planning

基　　金：国家自然科学基金项目(30560056;30860128);云南省中青年学术技术带头人后备人才培养计划(2005PY01);云南省教育厅科研基金资助(06Y1168;07Z11378)

摘　　要：目的:观察芸豆植物凝集素(PHA)对小鼠体外受精的影响及途径。方法:在含不同浓度芸豆PHA的HTF培养液中进行体外受精或用该培养液预处理小鼠卵母细胞再授精,观察其对小鼠体外受精的影响。结果:在含500、1000和5000μg/mlPHA培养液中,精子被完全凝集,受精率为0;用含500、1000和5000μg/mlPHA预处理卵母细胞后,加入获能精子,受精率为0;在含50和200μg/mlPHA预处理实验中,受精率为11.2%和5.9%。而在未经PHA处理空白对照实验中的卵母细胞体外受精率为81.3%,卵母细胞受精发育成囊胚的比率为48.4%,并且对照实验中产生的部分胚囊经胚胎移植产出正常的仔鼠。结论:芸豆PHA既可作用于精子,也可作用于卵子,最终可有效抑制小鼠受精。Objective：To explore the influence of phytohemagglutinin （PHA） extracted from Phaseolus vulgaris （white kidney bean） on in vitro fertilization in mice. Methods：Oocytes isolated from mice were treated with different concentrations of PHA. The fertilization rates in vitro were evaluated. Results：In PHA-contained media at concentration from 500 to 5000 μg/ml,all sperms were aggregated and their fertilization rates were 0; in 50μg/ml and 200μg/ml PHA containing media,fertilization rates were 11.2% and 5.9%,respectively. By contrast,the fertilization rate of the control was 81.3%. Among them,48.4% of fertilized oocytes developed into blastocysts and some offsprings were produced after embryo transfer. Conclusion：PHA from Phaseolus vulgaris is effective for the inhibition of in vitro fertilization in mice.

关 键 词：芸豆PHA 体外受精 抑制受精 小鼠 

分 类 号：R285.5[医药卫生—中药学]