急性重症胰腺炎湿热证动物模型建立初探  

Preliminary Study of Animal Modeling of Severe Acute Pancreatitis with Damp-Heat Syndrome

作　　者：齐文杰[1] 张淑文[1] 王红[1] 张苗苗[1] 文艳[1] 王宝恩[1] 

机构地区：[1]首都医科大学附属北京友谊医院感染暨急救医学科,北京100050

出　　处：《中国中医药信息杂志》2011年第2期47-50,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市中医管理局资助项目(JJ2008-015)

摘　　要：目的通过制作急性重症胰腺炎大鼠模型,探索急性热性病湿热证动物模型。方法采用5%的牛磺胆酸钠逆行胆胰管注入制作大鼠急性重症胰腺炎模型。观察造模后动物体重、体温、精神、大便、舌苔等,以舌苔厚腻作为评价动物模型湿热证存在的指标。大鼠随机分为模型组、假手术对照组、正常组。造模成功后模型组再根据舌苔是否厚腻分为舌苔厚腻组(A组)和舌苔正常组(B组)。进行血淀粉酶、内毒素、肿瘤坏死因子(TNF)测定及胰腺病理学分析。结果急性重症胰腺炎大鼠造模后出现倦怠、体重下降(P<0.05)、便溏、舌苔厚腻(出现频率42.6%)、内毒素及TNF水平升高,与假手术组及正常组比较,P<0.05,体温升高不明显。结论急性重症胰腺炎大鼠模型是一个更符合临床急性热性病湿热证发病过程的动物模型,为湿热证的证型实质探讨提供了较为理想的动物模型。Objective To explore animal modeling of acute fever disease with damp-heat syndrome by using rat models of severe acute pancreatitis （SAP）. Method SAP was induced by retrograde injection into the biliopancreatic duct of 5% sodium taurocholate. The body weight, temperature, mental status, stools, fur of tongue, etc. were observed after model made. A white or yellow and greasy tongue coating was considered as a marker to evaluate damp-heat syndrome of the animal model. Rats were divided into three groups randomly： SAP group, sham operated （SO） group and control （C） group. SAP group was divided further into A group （damp-heat syndrome group which a white or yellow and greasy tongue coating existed） and B group （normal tongue coating）. The level of serum amylase, endotoxin, TNF and the pathological lesion scores of pancreas tissue were analyzed. Results Lassitude, body weight loss （P 〈0.05）, loose stools and a white or yellow and greasy tongue coating （frequency of occurrence is 42.6%） were noted in parts of rat models. The level of plasma endotoxin and serum TNF were obviously increased （vs the group of SO and C, P〈0.05）. Body temperature of rat models were not significantly changed. Conclusion The SAP rat model can be considered as a good animal model in accordance with acute fever disease with damp-heat syndrome, which supplies a relative ideal animal model for exploring the pathogenesis of damp-heat syndrome.

关 键 词：动物模型 大鼠 湿热证 胰腺炎 

分 类 号：R285.5[医药卫生—中药学]