敦煌石室大宝胶囊对亚急性衰老大鼠脑功能的保护效应及作用机制研究  

Study on the Protective Function and Its Mechanism of Dunhuang Shishi Dabao Capsule on Brain Function of Subacutely Aging Rats

作　　者：程容[1] 成映霞[1] 段永强[1] 王道坤[1] 朱立鸣[1] 梁玉杰[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2011年第2期50-52,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省科技攻关计划(2GS064-A43-020-22);兰州市科技局计划项目

摘　　要：目的探讨敦煌石室大宝胶囊对亚急性衰老大鼠脑功能的保护效应及作用机制。方法除空白对照组外,采用D-半乳糖建立大鼠衰老模型并随机分为模型组、阳性药物组及敦煌石室大宝胶囊大、中、小剂量组,经药物干预后,测定各组大鼠脑组织去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、Na+-K+-ATPase、Ca2+-ATPase、Ca2+-Mg2+-ATPase的表达水平和Ca2+含量。结果与空白对照组比较,模型组大鼠脑组织内NE、DA、5-HT含量明显降低(P<0.05),Na+-K+-ATPase、Ca2+-ATPase、Ca2+-Mg2+-ATPase活性显著降低(P<0.01,P<0.05,P<0.01),Ca2+含量显著升高(P<0.05);应用受试药物治疗后,大鼠脑组织内NE、DA、5-HT含量显著增高(P<0.05),Na+-K+-ATPase、Ca2+-ATPase、Ca2+-Mg2+-ATPase活性显著增高(P<0.05或P<0.01),Ca2+含量显著降低(P<0.05)。结论敦煌石室大宝胶囊具有促进衰老大鼠脑组织单胺类神经递质表达以及调节脑组织钙稳态的作用,对老化大脑的功能有一定改善作用。Objective To study the protective function and its mechanism of Dunhuang Shishi Dabao Capsule （DHDB） on brain function of sub-acutely aging rats. Methods Except normal group, the subacutely aging model rats were made by injecting D-gal into abdominal cavity continually and divided into 5 groups randomly： model group, positive control group and high, middle, low dose of DHDB group. After treated with corresponding drugs, cerebral cortex NE, DA, 5-HT, Na＋-K＋-ATPase, Ca2＋-ATPase, Ca2＋-Mg2＋-ATPase and Ca2＋ levels were detected. Results NE, DA, 5-HT, Na＋-K＋-ATPase, Ca2＋-ATPase and Ca2＋-Mg2＋-ATPase levels in model group were significantly downregulated compared with normal group （P 〈0.05）, Ca2＋ level was increased significantly （P 〈 0.05）. After treated with DHDB, NE, DA, 5-HT, Na＋-K＋-ATPase, Ca2＋-ATPase and Ca2＋-Mg2＋-ATPase levels were significantly upregulated （P 〈0.05）, Ca2＋ level was reduced significantly （P〈0.05）. Conclusions DHDB can upregulate monoamine neurotransmitter levels and regulate calcium homeostasis in the brain tissue of aging model rats, and play an important role in improving the aging cerebral function.

关 键 词：单胺类神经递质 钙稳态 亚急性衰老模型 敦煌石室大宝胶囊 

分 类 号：R285.5[医药卫生—中药学]