高效液相色谱法测定狼疮灵颗粒中芍药苷的含量  

Determination of Paeoniflorin in Langchuangling Granules by HPLC

作　　者：孙川[1] 冯俭[2] 李煌[1] 

机构地区：[1]成都中医药大学药学院,四川成都610072 [2]成都中医药大学附属医院,四川成都610072

出　　处：《中国中医药信息杂志》2011年第2期66-67,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立狼疮灵颗粒中芍药苷的含量测定方法。方法采用高效液相色谱法,色谱柱:Hypersil ODS-2(150 mm×4.6 mm,5.0μm);流动相:乙腈-0.4%的磷酸水溶液(10∶90);检测波长:230 nm;流速:1.0 mL/min,柱温:25℃。结果芍药苷在0.608～3.04μg范围内呈良好的线性关系(r=0.999 9),加样回收率97.77%,RSD=0.62%(n=5)。结论该方法快速可行、简捷准确、重复性好、结果可靠,可用于控制狼疮灵颗粒的质量。Objective To establish an accurate method for determination of paeoniflorin in Langchuagrding Granules. Methods Hypersil ODS-2 （4.6 mm× 150 mm, 5.0 um ） in an oven at 25 ℃ was need, with a mobile phase consisting of acetonitril-0.4% granolite treatment （10 ： 90） and UV detector at 230 nm, the flow rate was 1.0 mL/min. Result The linear range of paeoniflorin was within 0.608-3.04 ug （r=0.9999）. The average recovery was 97.77% and RSD was 0.62% （n=5）. Conclusion The method is simple and accurate. It can be used for the quality control of Langchuagnling Granules.

关 键 词：狼疮灵颗粒 芍药苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]