诃子对乌头碱致大鼠心肌细胞损伤模型的保护作用  

Protective Effects of Decoction of Terminalia on Myocardial Cells Damaged by Aconitine

作　　者：潘燕[1] 张述禹[2] 李峰[3] 侯金凤[2] 周秀娟[2] 

机构地区：[1]北京大学医学部基础医学院药理学系,国家天然药物及仿生药物国家重点实验室,北京100191 [2]内蒙古医学院药学院,内蒙古呼和浩特010059 [3]首都医科大学北京神经科学研究所,教育部神经变性病学重点实验室,北京100069

出　　处：《中国民族医药杂志》2011年第1期43-46,共4页Journal of Medicine and Pharmacy of Chinese Minorities

摘　　要：目的:利用乌头碱建立大鼠心肌损伤模型,观察诃子对心肌细胞形态和心肌乳酸脱氢酶的保护作用。方法:用比色法测定心肌细胞内乳酸脱氢酶(LDH)、肌酸激酶(CK)的总活性;用电泳法测定心肌及血清中LDH同工酶的含量;用细胞化学HE染色法观察心肌病理结构的改变。结果:诃子高剂量组与模型组心肌CK酶活性有显著差异,P<0.05;诃子高、低剂量组血清LDH_1/LDH_2比值的异常百分率较模型组低。诃子高、低剂量组实验动物心肌细胞结构较模型组完整,其核形态规整,肌节清晰,肌原纤维排列整齐,横纹、闰盘趋于规则,接近正常。结论:本实验证实,蒙药诃子在心肌酶和形态学方面有保护心肌,缓解乌头碱对心肌毒性的作用。Objective：Explore the protective effects of decoction of terminalia（DT） on myocardial cells damaged by aconitine. Methods： Activities of lactate dehydrogenase （LDH） and creatine kinase （CK） and isoenzymes of LDH were monitored by chemicalmethods; Cytochemical technologies were used to detect the structures of myocardial cells. Resuits：Between high dosage of DT and model,there were difference in CK activity in myocardium（P 〈 0.05） .The abnormal percentage of LDH1/LDH2 reduced in high and lower dosages of DT groups. The myocardial structures of two dosages of DT groups were more complete and regular than model＇s. Conclusion： The Mongolia medicine, DT, has protective effect on myocardium and can relieve the toxicity of Aconitine.

关 键 词：诃子 心肌 组织细胞化学 乳酸脱氢酶 同工酶 

分 类 号：R291.2[医药卫生—民族医学]