尼纶线、薇荞线对缝合部位皮肤衰老因子影响的实验研究  

Experimental study for comparing the effects of nylon line and vicryl line on the skin aging factors on the operation area

作　　者：马恬[1] 贾赤宇[2] 张辉[3] 徐凯[3] 

机构地区：[1]军医进修学院,北京100853 [2]中国人民解放军第309医院整形美容烧伤修复中心科 [3]山西医科大学第二临床医学院

出　　处：《山西医科大学学报》2011年第1期37-41,共5页Journal of Shanxi Medical University

摘　　要：目的建立家兔的衰老模型,通过比较尼龙线和薇荞线对于手术区局部皮肤衰老因子影响的研究,以此来指导临床选择缝线。方法 20只衰老家兔随机分为尼龙线组和薇荞线组。将两种线植入家兔皮肤下,分别于3d,7d,15d,30d,45d,60d,90d切取各缝线手术区皮肤样本,一份测定家兔皮肤组织中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、过氧化氢(H2O2)及羟脯氨酸的含量,另一份做HE染色,观察手术区皮肤组织学的改变。结果薇荞线组总抗氧化能力、羟脯氨酸的含量、超氧化物歧化酶的活性数值均大于尼龙线组,两组间差异存在统计学意义(P<0.05)。薇荞线组过氧化氢的含量显著小于尼龙线组(P<0.05)。结论薇荞线植入部位比尼龙线植入部位总抗氧化能力数值高,超氧化物歧化酶活性高,过氧化氢含量减少,从而延缓皮肤组织的衰老;而羟脯氨酸的高含量可以使胶原蛋白的合成增加,从而延缓皮肤组织衰老。薇荞线可能通过改变手术区局部皮肤衰老因子从而更有利于伤口的愈合。Objective To explore a better clinical surgical suture by comparing the effect of nylon line and vicryl line on the skin aging on the operation area.Methods Twenty aging rabbits were randomly divided into 2 groups：nylon group and vicryl group.Two different kinds of lines were implanted under the skin of rabbits at day 1,30,45,60,75,83,87,respectively.At day 90,skin samples from surgical areas were cut and divided into two parts in two groups.One part of samples was used to determine total antioxidant capacity（T-AOC）,superoxide dismutase（SOD） activity,the contents of hydrogen peroxide（H2O2） and hydroxyproline（HYP）in aging skins,while the other part was used to observe the skin histopathological changes on the operation area.Results The total antioxidant capacity,the superoxide dismutase capacity,and the hydroxyproline content in vicryl group were significantly higher than those in nylon group（P〈0.05）.The level of hydrogen peroxide was significantly lower in vicryl group than that in nylon group（P〈0.05）.Conclusion Compared with the nylon line,the vicryl line can delay the skin aging by increasing the total antioxidant capacity and superoxide dismutase activity and decreasing the hydrogen peroxide in the implantation site.Compared with the nylon line,the vicryl line may be more favorable for wound healing by regulating the expression of skin aging factors in the operation region.

关 键 词：外科缝线 羟脯氨酸 超氧化物歧化酶 衰老 

分 类 号：R318.08[医药卫生—生物医学工程]