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机构地区:[1]广西医科大学附属第四医院,广西柳州545005
出 处:《国际检验医学杂志》2011年第1期25-25,28,共2页International Journal of Laboratory Medicine
基 金:广西壮族自治区卫生厅科研课题(Z2007276)
摘 要:目的比较高效液相色谱法(HPLC法)和微生物检定法测定奈替米星血药浓度。方法分别用HPLC法和微生物检定法测定血药浓度。结果 HPLC法平均回收率为97.87%,日内、日间相对标准偏差(RSD)分别为4.40%和5.49%;微生物检定法平均回收率为101.13%,日内、日间RSD分别为5.06%和6.88%。结论两种方法回收率和精密度基本一致,受试者血药浓度差异无统计学意义。Objective Two methods for determination of netilmicin in human plasma were compared. Methods HPLC method and microbial assay were respectively used to determine the netilmicin concentration in serum. Results The average recovery of HPLC method was 97.87 %, within-day and between-day RSD were 4.40% and 5.49 %. The average recovery of microbial assay was 101.13 %,within-day and between-day RSD were 5.06% and 6.88%. Conelusion The recovery and the precision of these methods were found to be similar. There were no significant difference in netilmicin serum concentrations by HPLC method and by the microbial assay.
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