一次注射^3H—Leu测定肉仔鸡内源氨基酸排泄量的方法学研究  

METHODOLOGICAL STUDY ON DETERMINING ENDOGENOUS AMINO ACID EXCRETION OF BROILER CHICKENS BY SINGLE INTRAVENOUS INJECTION OF 3HLEUCINE

作　　者：姚军虎[1,2] 王康宁[1,2] 杨凤[1,2] 周安国 蔡学林[1,2] 端木道 

机构地区：[1]四川农业大学动物营养研究所 [2]西北农业大学动物科学与医学学院

出　　处：《核农学报》1999年第4期229-235,共7页Journal of Nuclear Agricultural Sciences

摘　　要：给强饲无氮饲粮（ＮＦＤ）、ＮＦＤ＋３２０％酶解酪蛋白（ＥＨＣ）、以豆粕为唯一氮源粗蛋白（ＣＰ）为５％及２０％半合成饲粮的肉仔鸡，静脉注射３０μＣｉ／ｋｇ体重３ＨＬｅｕ，注射后５ｍｉｎ、４ｈ、２４ｈ、３６ｈ及４８ｈ测定血浆游离Ｌｅｕ比放射性强度（ＳＲｐ）及４８ｈ内排泄物比放射性强度（ＳＲ）。结果表明，不同饲粮排泄物内源Ｌｅｕ比放射性强度（ＳＲｅ）与ＳＲｐ定积分值之比恒等，证明我们提出的一次注射法可行。一次注射法检测的ＣＰ２０％饲粮时内源氨基酸总排泄量比ＮＦＤ高２０５０％。Fourty broiler chickens(1\^5kg of body weight,BW)were randomly divided into 20 groups.Every fifth group was force\|fed a nitrogen\|free diet(NFD) or a NFD+3\^20% enzyme hydrolysed casein(EHC) diet or diets with 5% and 20% crude protein(CP) in which soybean meal(sol.) was the sole nitrogen source. 30Ci 3H\|leucine/kg BW was intravenously injected into all birds just after the force\|feeding.Venous blood samples were taken at 5 min,4h,24h,36h and 48h after the injection,and the amount of excreta for the whole period of 48h was collected.The amino acids excreted after force\|feeding NFD+3\^20%EHC of CP5% diet were theoretically endogenous.The ratios of specfic radioactivity(SR) in excreta and the value of definite integral in free plasma from 0 to 48 h after injection of labelled leucine were not different(P>0\^05) when NFD,NFD+3\^20%EHC or CP5% diet was fed.From these results and theoretical analysis,it was suggested that for the birds with CP20% diet,the ratio of SR in endogenous leucine and value of definite integral in free plasma from 0 to 48 h after injection of labelled leucine would be the same as that of the birds with NFD diet,and thus endogenous losses of leucine and other amino acids,by the endogenous amino acid pattern measured with NFD diet,could be estimated for CP20% diet.The endogenous amino acid losses measured by this new technique was 120\^50% of those measured by NFD method.It was suggested that single intravenous injection of 3H\| leucine first proposed in the paper would be more valuable for determining endogenous amino acid losses,especially when practical nitrogen\|containing diet was fed.

关 键 词：肉仔鸡 一次注射法 内源氨基酸 测定 排泄量 

分 类 号：S831.1[农业科学—畜牧学]